Effect of treadmill exercise on vertebral and tibial bone mineral content and bone mineral density in the aged adult rat: Determined by dual energy X-ray absorptiometry
Effect of treadmill exercise on vertebral and tibial bone mineral content and bone mineral density in the aged adult rat: Determined by dual energy X-ray absorptiometry
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DOI：
10.1007/bf00298725
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发表时间：
1993-03
影响因子：
4.2
通讯作者：
J. Yeh;J. Aloia;J. Tierney;S. Sprintz
中科院分区：
文献类型：
--
作者：
J. Yeh;J. Aloia;J. Tierney;S. Sprintz
采用超高分辨率模式的双能 X 射线骨密度仪（DXA；Hologic QDR-1000W）检查每只 14 月龄雌性大鼠在研究第 0、9 和 16 周时胫骨/腓骨和椎骨 L4 +L5 骨矿物质含量 (BMC) 和骨矿物质密度 (BMD) 的变化。将20只大鼠通过分层体重法随机分为两组，对照组和运动组。锻炼包括在平板跑步机上跑步，每分钟 17 m，每天 1 小时，每周 5 天。与对照组相比，运动训练后9周胫骨/腓骨BMC和椎骨BMD明显增加（双因素方差分析P=0.014）。训练16周后，胫骨/腓骨BMC和BMD增加的斜率分别是对照组的9倍和5倍（曼-惠特尼检验分别P<0.01和P<0.05）。对于胫骨/腓骨和椎骨，切除骨的最终干重与完整大鼠的最终 BMC 之间的相关系数 (r) 分别为 0.843 和 0.71。总之，我们发现，在老年大鼠中，到 9 周时，运动会增加胫骨的 BMC 和 BMD，而在椎骨中，仅发现 BMD 增加。这项研究表明，通过考虑动物之间的个体差异以及组之间的变化，这种精确和准确的 DXA 技术可用于对大鼠体内骨矿物质随时间变化的纵向研究。
Dual energy X-ray absorptiometry (DXA; Hologic QDR-1000W) in an ultrahigh-resolution mode, was used to examine the changes in tibial/fibula and vertebral L4 +L5 bone mineral content (BMC) and bone mineral density (BMD) in each 14-month-old female rat at 0, 9, and 16 weeks of study. Twenty rats were randomized by a stratified weight method into two groups, control and exercised. Exercise consisted of running on a flat-bed treadmill, 17 m/minute, 1 hour/day and 5 days/week. As compared with the control group, a significant increase in tibia/fibula BMC and vertebral BMD was apparent at 9 weeks after exercise training (P=0.014 by 2-way analysis of variance). The slope of the gain of the tibia/fibula BMC and BMD by 16 weeks of training was ninefold and fivefold higher than that of the control group (P<0.01 andP<0.05, respectively, by Mann-Whitney test). The correlation coefficient (r) between the final dry weight of excised bone and the final BMC of the intact rat was 0.843 and 0.71 for tibia/fibula and vertebrae, respectively. In summary, we found that in the aged rat, by 9 weeks, exercise increases BMC and BMD in the tibia, whereas in the vertebrae, only increases in the BMD were found. This study demonstrates that this precise and accurate DXA technique is useful in a longitudinal study ofin vivobone mineral changes in the rat over time by taking into account the individual variation between animals as well as changes between groups.