NEUROPEPTIDE-Y DISTRIBUTION IN HUMAN-BRAIN
NEUROPEPTIDE-Y DISTRIBUTION IN HUMAN-BRAIN
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DOI：
10.1038/306584a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
POLAK, JM
中科院分区：
文献类型：
--
作者：
ADRIAN, TE;ALLEN, JM;POLAK, JM
Tatemoto和Mutt最近利用C端NH2基团的存在从猪脑1中鉴定和分离出一种新的多肽-神经肽Y(NPY)。这种36个氨基酸的多肽随后被证明在极低摩尔浓度的2-4中对离体输精管、血管平滑肌和胰腺腺泡细胞具有活性。鉴于这些强大的作用，我们现在用放射免疫分析和免疫细胞化学的方法研究了它在人脑中的分布。已发现高浓度的NPY，超过了迄今为止被认为是最丰富的神经肽的缩胆囊素和生长抑素。NPY的分布不同于所描述的任何其他多肽系统，尤其集中在基底节、杏仁核和锥状核。免疫细胞化学显示有大量的NPY神经元胞体，以尾状核和尾壳核最为明显。免疫反应阳性神经元胞体也明显定位于皮质区域，特别是第V层和第VI层。NPY是一种新发现的具有强大生物学活性的多肽，因此似乎是人类神经肽中含量最丰富的。基底节内大量的NPY神经元提示NPY在人类运动功能的调控中起着重要作用。
Tatemoto and Mutt recently used the presence of a C-terminal NH2group to identify and isolate a new peptide, neuropeptide Y (NPY), from porcine brain1. This 36 amino acid peptide was subsequently shown to be active on isolated vas deferens, vascular smooth muscle and pancreatic acinar cells in very low molar concentrations2–4. In view of these potent effects we have now investigated its distribution in the human brain by radio-immunoassay and immunocytochemistry. High concentrations of NPY have been found, exceeding those of cholecystokinin and somatostatin, hitherto considered to be the most abundant neuropeptides. The distribution of NPY was different from that of any other peptide system described, being particularly concentrated in the basal ganglia, amygdala and nucleus accum-bens. Immunocytochemistry demonstrated a large number of NPY neuronal cell bodies especially in the caudate and puta-men. Immunoreactive neuronal cell bodies were also clearly localized in cortical areas, particularly layers V and VI. NPY, a newly discovered peptide with potent biological activity, thus seems to be among the most abundant of human neuropeptides. The massive numbers of NPY neurones in the basal ganglia suggest NPY to be of fundamental importance in the control of human motor function.