Detection of a gene encoding a phosphatidylinositol‐specific phospholipase C that is co‐ordinately expressed with listeriolysin in Listeria monocytogenes
Detection of a gene encoding a phosphatidylinositol‐specific phospholipase C that is co‐ordinately expressed with listeriolysin in Listeria monocytogenes
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检测编码磷脂酰肌醇特异性磷脂酶 C 的基因，该基因与单核细胞增生李斯特菌中的李斯特菌溶血素协同表达
DOI：
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发表时间：
1991
影响因子：
3.6
通讯作者：
T. Chakraborty
中科院分区：
文献类型：
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作者：
M. Leimeister;E. Domann;T. Chakraborty
一种磷脂酰肌醇特异性磷脂酶C(PI-PLC)已被检测到，它是致病李斯特菌种单核细胞增多性李斯特菌和伊万诺维氏李斯特菌所特有的。对表达PI-PLC活性的重组大肠杆菌进行的缺失分析和Maxicell分析表明，该活性与一个34 kDa的多肽有关。核苷酸序列分析表明，编码该多肽的基因由317个氨基酸残基组成，含有一个22个氨基酸的信号肽。这个基因被命名为PIC，即磷脂酰肌醇特异性磷脂酶C，它与李斯特溶素基因背靠背地位于单核细胞增生性李斯特菌的染色体上，这些基因由不同的非重叠启动子转录。PIC基因的表达依赖于PrFA基因的产物，PrFA基因也调节着李斯特菌溶素基因在单核细胞增多性李氏杆菌中的表达。
A phosphatidylinositol‐specific phospholipase C (PI‐PLC) that is unique to the pathogenic Listeria species L. monocytogenes and L. ivanovii has been detected. Deletion analysis performed with Escherichia coli recombinants expressing PI‐PLC activity together with maxicell analysis showed that a 34kDa polypeptide was responsible for this activity. Nucleotide sequencing revealed that the gene encoding this polypeptide comprises 317 amino acid residues with a 22‐amino‐acid signal peptide. This gene, designated pic for phosphatidylInositol‐specific phospholipase C, is located back to back with the listeriolysin gene on the chromosome of L. monocytogenes where these genes are transcribed by divergent non‐overlapping promoters. Expression of the pic gene is dependent on the product of the prfA gene, which also regulates expression of the listeriolysin gene in L. monocytogenes.