Population Dynamics of Iron-Oxidizing Communities in Pilot Plants for the Treatment of Acid Mine Waters
Population Dynamics of Iron-Oxidizing Communities in Pilot Plants for the Treatment of Acid Mine Waters
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DOI：
10.1021/es900067d
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发表时间：
2009-08-15
影响因子：
11.4
通讯作者：
Seifert, Jana
中科院分区：
文献类型：
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作者：
Heinzel, Elke;Janneck, Eberhard;Seifert, Jana
通过对两个矿山酸性水处理中试装置的铁氧化微生物群落进行监测，考察了pH、铁浓度和停留时间等工艺参数对系统稳定性的影响，以评价该处理技术在工业规模上的适用性。使用T-RFLP（末端限制性片段长度多态性）在几个月的时间内跟踪微生物种群的动态。为了更精确的定量，开发了两种针对两个突出组的TaqMan测定，并通过实时PCR验证了这些类群在铁氧化群落中的相对丰度。调查显示，铁氧化社区明显占主导地位的两组Betaproteobacteria隶属于知之甚少，尚未认识到的物种“Ferrovum myxofaciens”和菌株相关的铁褐盖菌，分别。这些类群占主导地位的微生物群落在整个调查期间，加速了亚铁的氧化，尽管不断变化的特点，矿山沃茨流入植物。因此，可以假定，处理技术也可应用于其他矿址，而且这些生物在这种处理系统中发挥着关键作用。
The iron-oxidizing microbial community in two pilot plants for the treatment of acid mine water was monitored to investigate the influence of different process parameters such as pH, iron concentration, and retention time on the stability of the system to evaluate the applicability of this treatment technology on an industrial scale. The dynamics of the microbial populations were followed using T-RFLP (terminal restriction fragment length polymorphism) over a period of several months. For a more precise quantification, two TaqMan assays specific for the two prominent groups were developed and the relative abundance of these taxa in the iron-oxidizing Community was verified by real-time PCR. The investigations revealed that the iron-oxidizing community was clearly dominated by two groups of Betaproteobacteria affiliated with the poorly known and not yet recognized species "Ferrovum myxofaciens" and with strains related to Gallionella ferruginea, respectively. These taxa dominated the microbial community during the whole investigation period and accelerated the oxidation of ferrous iron despite the changing characteristics of mine waters flowing into the plants. Thus, it is assumed that the treatment technology can also be applied to other mine sites and that these organisms play a crucial role in such treatment systems.