Anode modification to improve the performance of a microbial fuel cell volatile fatty acid biosensor
Anode modification to improve the performance of a microbial fuel cell volatile fatty acid biosensor
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DOI：
10.1016/j.snb.2014.04.062
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发表时间：
2014-10-01
影响因子：
8.4
通讯作者：
Premier, Giuliano C.
中科院分区：
文献类型：
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作者：
Kaur, Amandeep;Ibrahim, Saad;Premier, Giuliano C.
开发一种简单且低成本的传感器，例如用于测量短链挥发性脂肪酸（VFA）的微生物燃料电池（MFC），将使这一重要的生物过程参数得以实施。然而，使用微生物燃料电池（MFC）作为传感器需要阳极处持续的微生物生物催化。这项研究将碳/导电聚合物复合电极作为一种机制，以确保传感器信号具有时间稳定性、可重复性以及对乙酸、丙酸和丁酸浓度变化的快速响应时间。细菌的固定化：用功能化聚吡咯涂层修饰碳表面，增加并保持电极上带负电的细菌总数；或者通过使用天然聚合物和覆盖预适应微生物群落的介质来改善催化作用并保护它们免受样品基质的影响。对六种不同的天然聚合物和/或电聚合物阳极配置进行了比较，发现聚（吡咯烷基铵）可加速基于 MFC 的传感器的启动，并提供改进的稳定性、可重复性和恢复更短的信号响应。 (C) 2014 Elsevier B.V. 保留所有权利。
The development of a simple and low cost sensor such as a microbial fuel cell (MFC) to measure short chain volatile fatty acids (VFAs) would enable the implementation of this important bioprocess parameter. However using microbial fuel cells (MFCs) as transducers requires consistent microbial biocatalysis at the anode. This study considers carbon/conductive polymer composite electrodes as a mechanism to ensure the sensor signal has temporal stability, repeatability and a short response time to variations in concentration of acetic, propionic and butyric acid. The immobilization of bacteria by; modifying the carbon surface with functionalized poly(pyrrole) coatings, increasing and holding the total number of negatively charged bacteria on the electrode; or by using natural polymers with mediators covering the pre-acclimated microbial community to improve catalytic action and to protect them from the sample matrix was investigated. Six different natural polymers and/or electropolymers anode configurations were compared and it was found that poly(pyrrole-alkyl ammonium) accelerates start-up of MFC based sensors and provided improved stability, repeatability and recovery shorter signal response. (C) 2014 Elsevier B.V. All rights reserved.