Allogenic tooth transplantation inhibits the maintenance of dental pulp stem/progenitor cells in mice
Allogenic tooth transplantation inhibits the maintenance of dental pulp stem/progenitor cells in mice
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DOI：
10.1007/s00441-014-1818-8
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发表时间：
2014-03
影响因子：
3.6
通讯作者：
Kotaro Saito;M. Nakatomi;S. Kenmotsu;H. Ohshima
中科院分区：
文献类型：
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作者：
Kotaro Saito;M. Nakatomi;S. Kenmotsu;H. Ohshima
我们最近的研究表明，同种异体牙移植可能会影响牙髓干细胞/祖细胞的维持。本研究旨在通过BrdU追踪、BrdU、巢蛋白和Ki67免疫组化、原位杂交forDspp、透射电镜和TUNEL实验，探讨同种异体移植物对小鼠牙齿再植和同种异体或自体牙移植后牙髓干/祖细胞维持的影响。brdu标记的动物拔除上颌第一磨牙后，立即将牙齿重新定位在原牙槽中，或者在未标记或相同的动物中切除牙根并立即允许或自动移植到舌下区域。在对照组中，两种类型的BrdU标签保留细胞（lrc）分布在牙髓中：致密的BrdU反应细胞和颗粒状的BrdU反应细胞。在再植和自体移植中，致密的lrc在整个实验期间都保持在牙髓中心，与血管周围环境相关，具有增殖能力，并保持向成牙细胞样细胞或成纤维细胞的分化能力。相比之下，同种异体移植物在术后第4周时，牙髓组织中心的lrc消失。lrc消失的原因是，即使在没有免疫排斥反应的情况下，除了新分化的成牙细胞样细胞外，lrc也会发生广泛的凋亡。结果表明，同种异体移植物中的宿主和受体相互作用扰乱了密集lrc（可能是干细胞/祖细胞）的维持，导致这些细胞类型消失。
Our recent study suggested that allogenic tooth transplantation may affect the maintenance of dental pulp stem/progenitor cells. This study aims to elucidate the influence of allograft on the maintenance of dental pulp stem/progenitor cells following tooth replantation and allo- or auto-genic tooth transplantation in mice using BrdU chasing, immunohistochemistry for BrdU, nestin and Ki67, in situ hybridization forDspp, transmission electron microscopy and TUNEL assay. Following extraction of the maxillary first molar in BrdU-labeled animals, the tooth was immediately repositioned in the original socket, or the roots were resected and immediately allo- or auto-grafted into the sublingual region in non-labeled or the same animals. In the control group, two types of BrdU label-retaining cells (LRCs) were distributed throughout the dental pulp: those with dense or those with granular reaction for BrdU. In the replants and autogenic transplants, dense LRCs remained in the center of dental pulp associating with the perivascular environment throughout the experimental period and possessed a proliferative capacity and maintained the differentiation capacity into the odontoblast-like cells or fibroblasts. In contrast, LRCs disappeared in the center of the pulp tissue by postoperative week 4 in the allografts. The disappearance of LRCs was attributed to the extensive apoptosis occurring significantly in LRCs except for the newly-differentiated odontoblast-like cells even in cases without immunological rejection. The results suggest that the host and recipient interaction in the allografts disturbs the maintenance of dense LRCs, presumably stem/progenitor cells, resulting in the disappearance of these cell types.