Carboxylicivirga flava sp. nov., isolated from marine surface sediment.
Carboxylicivirga flava sp. nov., isolated from marine surface sediment.
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DOI：
10.1099/ijsem.0.001533
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发表时间：
2016-12
影响因子：
2.8
通讯作者：
Hui Wang;Cancan Qi;Weiwei Chen;Wenwen Dong;Haitian Tang;Xiaoke Hu
中科院分区：
文献类型：
--
作者：
Hui Wang;Cancan Qi;Weiwei Chen;Wenwen Dong;Haitian Tang;Xiaoke Hu
从中国渤海海域的沉积物中分离到一株新的细菌，命名为Q15T，并进行了多相分类研究。菌株Q15T的细胞是革兰氏阴性的，严格的需氧菌棒，产生圆形、扁平、橙色的菌落。基于16S rRNA基因序列的系统发育分析表明，Q15T属于拟杆菌门Marinilabiliaceae中的Carboxylicivirga属。Q15T菌株与该属3个已知种的模式菌株(Carboxylicivirga taeanensis MEBiC 08903T、Carboxylicivirga Mesica MEBiC 07026T和Carboxylicivirga Linearis FB218T)的16S rRNA基因序列相似性为94.0-95.2%。菌株Q15T的DNA G+C含量为44.7mol%。细胞内脂肪酸组成以iso-C15：0、Anteo-C15：0和iso-C17：03-OH为主，呼吸苯二酚主要为菜籽酮MK-7。极性脂包括磷脂酰乙醇胺、一种未知的氨基脂、一种未知的磷脂和其他未知的脂类。根据目前多相分析的数据，发现了一新种--黄色羧基病毒属(Carboxylicivirga Huang)。，以Q15T(=CICC 23923T=KCTC 42707T)为模式菌株。
A novel bacterial strain, designated Q15T, was isolated from sediments obtained from the Bohai Sea in China and subjected to a polyphasic taxonomic study. Cells of strain Q15T were Gram-stain-negative, strictly aerobic rods that produced circular, flat, orange colonies. Phylogenetic analysis based on 16S rRNA gene sequences revealed that Q15T was affiliated with the genus Carboxylicivirga in the family Marinilabiliaceae of the phylum Bacteroidetes. Strain Q15T differed genotypically from the type strains of the three recognized species of this genus (Carboxylicivirga taeanensis MEBiC 08903T, Carboxylicivirga mesophila MEBiC 07026T and Carboxylicivirga linearis FB218T) and shared 94.0-95.2 % 16S rRNA gene sequence similarity with them. The DNA G+C content of strain Q15T was 44.7 mol%. The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0 3-OH, and menaquinone MK-7 was the main respiratory quinone. Polar lipids contained phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and other unknown lipids. Based on the data from the current polyphasic analysis, a novel species, Carboxylicivirga flava sp. nov., is hereby proposed with Q15T (=CICC 23923T=KCTC 42707T) as the type strain.