Porous three-dimensional scaffolds made of mineralised collagen:: Preparation and properties of a biomimetic nanocomposite material for tissue engineering of bone
Porous three-dimensional scaffolds made of mineralised collagen:: Preparation and properties of a biomimetic nanocomposite material for tissue engineering of bone
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DOI：
10.1016/j.cej.2007.09.029
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发表时间：
2008-03-15
影响因子：
15.1
通讯作者：
Koenig, U.
中科院分区：
文献类型：
--
作者：
Gelinsky, M.;Welzel, P. B.;Koenig, U.
为了修复骨缺损并作为组织工程的基质，需要多孔支架，这些支架可以在实验室中轻松预接种细胞，并在植入后被组织入侵。我们已经开发出由矿化胶原蛋白I型-a纳米复合材料组成的多孔3D支架，其模拟健康骨组织的细胞外基质的组成。详细介绍了材料加工的步骤以及该生物材料的物理化学、结构和力学性能。差示扫描量热技术，这提供了一个很好的可能性，研究胶原基材料，被用来在原位的最终支架及其所有中间体。此外，具有约200 μ m孔径的支架的互连孔隙度已被证明非常适合于均质细胞接种，这用人骨髓基质细胞证明。(C)2007 Elsevier B. V.保留所有权利。
For healing of bone defects and as matrix for tissues engineering, porous scaffolds are required that can be easily pre-seeded with cells in the lab and invaded by tissue after implantation. We have developed porous 3D scaffolds consisting of mineralised collagen type I-a nanocomposite, which mimics the composition of extracellular matrix of healthy bone tissue. The steps of material processing as well as the physico-chemical, structural and mechanical properties of this biomaterial are described in detail. The technology of differential scanning calorimetry, which provides an excellent possibility to investigate collagen-based materials, was used to characterise the final scaffold and all its intermediates in situ. Furthermore, the interconnecting porosity of the scaffolds with pore diameters of about 200 mu m has been shown to be highly suitable for homogenous cell seeding, demonstrated with human marrow stromal cells. (C) 2007 Elsevier B.V. All rights reserved.