Overexpression of SlRBZ Results in Chlorosis and Dwarfism through Impairing Chlorophyll, Carotenoid, and Gibberellin Biosynthesis in Tomato.
Overexpression of SlRBZ Results in Chlorosis and Dwarfism through Impairing Chlorophyll, Carotenoid, and Gibberellin Biosynthesis in Tomato.
复制标题
SlRBZ 的过度表达通过损害番茄中的叶绿素、类胡萝卜素和赤霉素生物合成而导致褪绿和矮化。
DOI：
10.3389/fpls.2016.00907
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发表时间：
2016
影响因子：
5.6
通讯作者：
Ye Z
中科院分区：
文献类型：
--
作者：
Fan M;Gao S;Ren J;Yang Q;Li H;Yang C;Ye Z
zfp在植物发育、胁迫响应、激素响应等诸多生物过程中发挥着重要作用。ranbp2型锌指转录因子已在动物和人类中得到鉴定。然而，它们在植物中的功能在很大程度上仍然未知。本研究在番茄中鉴定了一个ranbp2型锌指蛋白基因（SlRBZ）。SlRBZ在根、茎、叶、花和果实中均有组成性表达。SlRBZ-GFP融合蛋白定位于细胞核内。SlRBZ的过表达导致番茄的萎黄和矮化表型。生理指标测定表明，转基因植株的叶绿素、类胡萝卜素和GAs含量明显降低。此外，qRT-PCR和RNA-Seq分析表明，SlRBZ-OE植物中参与这些生物合成途径的基因转录明显减少。透射电镜超微结构观察表明，SlRBZ-OE植株质体不能发育成具有正常叶绿体膜和类囊体膜系统的成熟叶绿体。结果表明，SlRBZ的过表达可能通过阻断叶绿体发育而影响叶绿素、类胡萝卜素和赤霉素的生物合成，导致番茄萎黄和矮化。
ZFPs play important roles in many biological processes, including plant development, stress response, and phytohormone response. RanBP2-type zinc finger transcription factors have been characterized in animals and humans. However, their functions remain largely unknown in plants. In this study, we identified a RanBP2-type zinc finger protein gene (SlRBZ) in tomato. SlRBZ was constitutively expressed in roots, stems, leaves, flowers, and fruits. The SlRBZ-GFP fused protein was localized in the nucleus. Overexpression of SlRBZ resulted in chlorosis and dwarf phenotypes in tomato. Determination of physiological index showed that chlorophyll, carotenoid, and GAs contents were evidently decreased in transgenic plants. Furthermore, the qRT-PCR and RNA-Seq analyses demonstrated that the transcription of the genes involved in these biosynthesis pathways obviously decreased in SlRBZ-OE plants. In addition, ultrastructural observation by transmission electron microscopy indicated that plastids could not develop into mature chloroplasts with normal chloroplast membrane and thylakoid membrane system in SlRBZ-OE plants. The results suggest that overexpression of SlRBZ may impair the biosynthesis of chlorophyll, carotenoid, and gibberellin through blocking chloroplast development, resulting in chlorosis and dwarfism in tomato.