Comparative study on gastrointestinal microbiota of eight fish species with different feeding habits
Comparative study on gastrointestinal microbiota of eight fish species with different feeding habits
复制标题
8种不同食性鱼类胃肠道微生物群比较研究
DOI：
10.1111/jam.12663
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发表时间：
2014-12-01
影响因子：
4
通讯作者：
Yan, Q.
中科院分区：
文献类型：
--
作者：
Li, J.;Ni, J.;Yan, Q.
目的探讨鱼类基因型、食性和血清生理指标对肠道菌群组成的影响，以8种具有4种不同食性的鱼类为研究对象，采用高通量测序技术（454平台）对16 SrRNA基因V1 ~ V3区序列进行分析，比较不同鱼类肠道菌群组成。从48个样本中共获得551995条高质量序列，平均长度为463 bp。不同食性鱼类的OTU检测序列间差异不显著（One-way anova，F=1003，P=0400），但不同食性鱼类OTU数量间差异显著（One-way anova，F=7564，P
AimsTo reveal the effects of fish genotype, feeding habits and serum physiological index on the composition of gastrointestinal microbiota, eight fish species with four different feeding habits were investigated.Methods and ResultsThe V1 to V3 regions of 16S rRNA gene were analysed by high-throughput sequencing (454 platform) to compare the gut microbiota of different fish species. A total of 551995 high-quality sequences with an average length of 463bp were obtained from the 48 samples. No significant difference was observed among the detected sequences obtained from fishes with different feeding habits (One-way anova, F=1003, P=0400), but the number of OTUs among different feeding habits was significantly different (One-way anova, F=7564, P