Kinetics of relaxation from rigor of permeabilized fast-twitch skeletal fibers from the rabbit using a novel caged ATP and apyrase.
Kinetics of relaxation from rigor of permeabilized fast-twitch skeletal fibers from the rabbit using a novel caged ATP and apyrase.
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使用新型笼式 ATP 和腺苷三磷酸双磷酸酶，对兔子的透化快肌骨骼纤维从严酷状态进行放松的动力学。
DOI：
10.1016/s0006-3495(94)80730-1
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发表时间：
1994
影响因子：
3.4
通讯作者：
M. Ferenczi
中科院分区：
文献类型：
--
作者：
H. Thirlwell;J. Corrie;G. P. Reid;D. Trentham;M. Ferenczi
在快收缩透化兔肌纤维中，当它们从僵直状态松弛时，使用ATP的光化学产生，研究了张力衰减的复杂时间过程。已经使用了一种新的笼状ATP化合物，ATP的P3- 3 '，5'-二甲氧基苯偶姻酯（DMB-笼状ATP），以及ATP的P3-1-（2-硝基苯基）乙酯（NPE-笼状ATP）。DMB笼状ATP的光解速度比NPE笼状ATP快至少三个数量级。ADP对僵直松弛动力学的作用通过使用腺苷三磷酸双磷酸酶从僵直肌肉溶液中去除ADP来检查。从Pi对弛豫的影响研究了Pi敏感态的存在。改变僵硬张力，以检查张力对松弛的影响。与NPE笼状ATP相比，释放的ATP浓度大于0.7 mM的光解的时间过程中的松弛是更快的。在松弛张力记录的复杂性是由ADP引起的。在没有ADP的情况下，张力衰减单调后，光化学释放ATP的过程中，其速率不受Pi。在ADP的存在下，松弛更复杂，张力通过最大值。一个模型调用合作的相互作用，包括ADP含肌球蛋白头提供了一个合理的数据描述。
The complex time course of tension decay was investigated in fast-twitch permeabilized rabbit muscle fibers when they were relaxed from the rigor state using photochemical generation of ATP. A novel caged ATP compound, the P3–3',5'-dimethoxybenzoin ester of ATP (DMB-caged ATP), as well as the P3–1-(2-nitrophenyl)ethyl ester of ATP (NPE-caged ATP), have been used. DMB-caged ATP photolyzes at least three orders of magnitude more rapidly than NPE-caged ATP. The role of ADP on relaxation kinetics from rigor was examined by using apyrase to remove ADP from the rigor muscle solutions. The presence of Pi-sensitive states was investigated from the effect of Pi on relaxation. Rigor tension was varied enabling the influence of tension on the relaxation to be examined. The time course of relaxation was faster with DMB-caged ATP compared with NPE-caged ATP for concentrations of ATP released by photolysis greater than 0.7 mM. Most of the complexity in the relaxation tension records was caused by ADP. In the absence of ADP, tension decayed monotonically after photochemical release of ATP in a process whose rate was unaffected by Pi. In the presence of ADP, relaxation was more complex and tension passed through a maximum. A model invoking cooperative interactions involving ADP-containing myosin heads provides a reasonable description of the data.
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DOI：
10.1152/ajpcell.1993.265.1.c279
发表时间：
1993-07
期刊：
The American journal of physiology
影响因子：
--
作者：
Y. Luo;R. Cooke;E. Pate
通讯作者：
Y. Luo;R. Cooke;E. Pate
肌动球蛋白亚片段 1 ATP 酶的调节机制。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rosenfeld,SS;Taylor,EW
通讯作者：
Taylor,EW
1-N6-乙烯腺苷二磷酸从受调节的肌动球蛋白亚片段 1 中解离。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rosenfeld,SS;Taylor,EW
通讯作者：
Taylor,EW
激光诱导 ATP 光生成：研究肌肉收缩化学动力学的新方法。
DOI：
10.1016/b978-0-12-152824-9.50038-1
发表时间：
1984
期刊：
Current topics in cellular regulation
影响因子：
--
作者：
Hibberd,MG;Goldman,YE;Trentham,DR
通讯作者：
Trentham,DR