Human keratinocytes migrate to the negative pole in direct current electric fields comparable to those measured in mammalian wounds.
Human keratinocytes migrate to the negative pole in direct current electric fields comparable to those measured in mammalian wounds.
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DOI：
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发表时间：
1996
影响因子：
4
通讯作者：
K. Y. Nishimura;R. Isseroff;R. Nuccitelli
中科院分区：
文献类型：
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作者：
K. Y. Nishimura;R. Isseroff;R. Nuccitelli
先前对豚鼠皮肤伤口附近的横向电场的测量已经检测到伤口边缘附近的100-200 mV/mm之间的DC场。我们已经研究了迁移的原代人角质形成细胞在胶原蛋白基质上的易位反应，同时暴露于类似的生理直流电场。我们发现，角质形成细胞随机迁移胶原在5 mV/mm或更小的领域，但在更大的领域，他们向该领域的负极迁移，表现出趋电性。由于这些单元具有50微米的平均单元长度，这意味着它们能够检测沿着其长度的低至0.5 mV的电压梯度。这种cathodally定向运动表现出增加的方向性与10和100 mV/mm之间的场强增加。我们观察到一个最大的定向反应在100 mV/mm的细胞响应的字段在14分钟内的一半。在50 mV/mm以上的电场中，平均迁移速度往往大于较小的电场。我们的结论是，人类角质形成细胞迁移到负极性的直流电场是相同的幅度，在哺乳动物皮肤伤口附近的体内测量。
Previous measurements of the lateral electric fields near skin wounds in guinea pigs have detected DC fields between 100-200 mV/mm near the edge of the wound. We have studied the translocation response of motile primary human keratinocytes migrating on a collagen substrate while exposed to similar physiological DC electric fields. We find that keratinocytes migrate randomly on collagen in fields of 5 mV/mm or less, but in larger fields they migrate towards the negative pole of the field, exhibiting galvanotaxis. Since these cells have an average cell length of 50 microns, this implies that they are able to detect a voltage gradient as low as 0.5 mV along their length. This cath-odally-directed movement exhibits increased directedness with increasing field strengths between 10 and 100 mV/mm. We observe a maximally directed response at 100 mV/mm with half of the cells responding to the field within 14 minutes. The average speed of migration tended to be greater in fields above 50 mV/mm than in smaller fields. We conclude that human keratinocytes migrate towards the negative pole in DC electric fields that are of the same magnitude as measured in vivo near wounds in mammalian skin.