A fission-yeast gene encoding a protein with features of protein-tyrosine-phosphatases.
A fission-yeast gene encoding a protein with features of protein-tyrosine-phosphatases.
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裂殖酵母基因编码具有蛋白质酪氨酸磷酸酶特征的蛋白质。
DOI：
10.1073/pnas.88.8.3455
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发表时间：
1991
影响因子：
11.1
通讯作者：
Erikson,RL
中科院分区：
文献类型：
--
作者：
Ottilie,S;Chernoff,J;Hannig,G;Hoffman,CS;Erikson,RL
以编码哺乳动物蛋白酪氨酸磷酸酶（PTPases）保守序列的简并寡核苷酸探针为模板，采用聚合酶链反应（PCR）方法从粟酒裂殖酵母cDNA文库中扩增DNA片段。克隆的PCR产物预测的肽序列与PTPases中发现的肽序列相似，但与任何已发表的序列不相同。色葡萄用此PCR探针从cDNA文库中鉴定pyp1+基因，克隆并测序。该基因的序列预测了一个550个氨基酸的蛋白质，Mr 61，586，其中包括在哺乳动物PTPases中高度保守的氨基酸序列。pyp1+基因的破坏导致活细胞。pyp1+基因在S. pombe允许检测表观Mr为63，000的蛋白质。
Degenerate oligonucleotide probes encoding sequences conserved among mammalian protein-tyrosine-phosphatases (PTPases) were used to amplify DNA fragments from a Schizosaccharomyces pombe cDNA library by polymerase chain reaction (PCR) methods. A cloned PCR product predicted peptide sequences similar to those found in PTPases but not identical to any published sequences. A S. pombe gene, designated pyp1+, was identified in a cDNA library with this PCR probe, cloned, and sequenced. The sequence of the gene predicted a 550-amino acid protein with Mr 61,586, which includes amino acid sequences that are highly conserved in mammalian PTPases. Disruption of the pyp1+ gene resulted in viable cells. Overexpression of the pyp1+ gene in S. pombe permitted detection of a protein of apparent Mr 63,000.