Functionalized surface-charge SiO2 nanoparticles induce pro-inflammatory responses, but are not lethal to Caco-2 cells.
Functionalized surface-charge SiO2 nanoparticles induce pro-inflammatory responses, but are not lethal to Caco-2 cells.
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DOI：
10.1021/acs.chemrestox.9b00478
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发表时间：
2020-04
影响因子：
4.1
通讯作者：
S. Tada‐Oikawa;Mana Eguchi;M. Yasuda;Kiyora Izuoka;Akihiko Ikegami;S. Vranic;S. Boland;L. Tran;G. Ichihara;S. Ichihara
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文献类型：
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作者：
S. Tada‐Oikawa;Mana Eguchi;M. Yasuda;Kiyora Izuoka;Akihiko Ikegami;S. Vranic;S. Boland;L. Tran;G. Ichihara;S. Ichihara
纳米颗粒广泛应用于食品中，对其潜在的胃肠道毒性进行分析是必要的。本研究旨在确定二氧化硅（SiO2），二氧化钛（TiO 2）和氧化锌（ZnO）纳米颗粒对培养的THP-1单核细胞衍生的巨噬细胞和人上皮结直肠腺癌（Caco-2）细胞的影响。在THP-1巨噬细胞和Caco-2细胞中，暴露于ZnO NPs 24小时增加了氧化还原反应物质（ROS）的产生，并以剂量依赖性方式降低了细胞活力。虽然TiO 2和SiO2纳米颗粒诱导氧化应激，但它们对这两种细胞类型没有表现出明显的细胞毒性。使用具有中性、正或负表面电荷的荧光标记的SiO2 NPs研究了功能化SiO2 NPs对未分化和分化的Caco-2细胞的影响。两种类型的细胞暴露于三种SiO2纳米颗粒显着增加了它们的相互作用，在剂量依赖性的方式。与两种类型的细胞的最大相互作用是注意到暴露于更多的负表面电荷的SiO2纳米颗粒。暴露于正或负，但不是中性的，表面带电的SiO2纳米颗粒增加了分化的Caco-2细胞中的NO水平。分化的Caco-2细胞暴露于带正或负表面电荷的SiO2纳米颗粒也上调白细胞介素-8的表达。我们得出结论，功能化的表面电荷SiO2纳米颗粒可以诱导促炎反应，但无细胞毒性。
Nanoparticles (NPs) are widely used in food and analysis of their potential gastrointestinal toxicity is necessary. The present study was designed to determine the effects of silica dioxide (SiO2), titanium dioxide (TiO2), and zinc oxide (ZnO) NPs on cultured THP-1 monocyte-derived macrophages and human epithelial colorectal adenocarcinoma (Caco-2) cells. Exposure to ZnO NPs for 24 hours increased the production of redox response species (ROS) and reduced cell viability in a dose-dependent manner in THP-1 macrophages and Caco-2 cells. Although TiO2 and SiO2 NPs induced oxidative stress, they showed no apparent cytotoxicity against both cell types. The effects of functionalized SiO2 NPs on undifferentiated and differentiated Caco-2 cells were investigated using fluorescently-labeled SiO2 NPs with neutral, positive or negative surface-charge. Exposure of both types of cells to the three kinds of SiO2 NPs significantly increased their interaction in a dose-dependent manner. The largest interaction with both types of cells was noted with exposure to more negatively surface-charged SiO2 NPs. Exposure to either positively or negatively, but not neutrally, surface-charged SiO2 NPs increased NO levels in differentiated Caco-2 cells. Exposure of differentiated Caco-2 cells to positively or negatively surface-charged SiO2 NPs also upregulated interleukin-8 expression. We conclude that functionalized surface-charge SiO2 NPs can induce pro-inflammatory responses but are non-cytotoxic.