Factors controlling anaerobic ammonium oxidation with nitrite in marine sediments
Factors controlling anaerobic ammonium oxidation with nitrite in marine sediments
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DOI：
10.1128/aem.68.8.3802-3808.2002
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发表时间：
2002-08-01
影响因子：
4.4
通讯作者：
Thamdrup, B
中科院分区：
文献类型：
--
作者：
Dalsgaard, T;Thamdrup, B
在波罗的海-北海过渡区斯卡格拉克海沉积物中，探讨了硝酸盐和亚硝酸盐厌氧氧化铵的控制因素。在缺氧孵育与添加亚硝酸盐，约65%的氮气形成是由于厌氧氨氧化与亚硝酸盐，与其余的反硝化产生的。亚硝酸盐厌氧氨氧化反应表现出生物温度响应，最适速率为15 ℃，最高温度为37 ℃。该过程的生物学性质和亚硝酸盐和铵之间反应的1：1化学计量比表明，转化可能归因于厌氧氨氧化过程。试图在这种沉积物中找到其他厌氧氨氧化过程失败了。亚硝酸盐消耗的表观K值小于3 μ M，并且亚硝酸盐氨氧化和氮气生产反硝化的相对重要性与亚硝酸盐浓度无关。因此，定量的重要性，铵氧化亚硝酸盐在罐培养在升高的亚硝酸盐浓度可能代表了原位的情况。随着硝酸盐的加入，从硝酸盐产生亚硝酸盐的速度比其消耗快四倍，因此不会限制铵氧化的速率。因此，无论添加硝酸盐还是亚硝酸盐作为电子受体，该过程的速率都是相同的。有机物的加入并没有刺激反硝化作用，可能是因为它被淘汰锰还原或因为运输限制被删除，由于均匀化的沉积物。
Factors controlling the anaerobic oxidation of ammonium with nitrate and nitrite were explored in a marine sediment from the Skagerrak in the Baltic-North Sea transition. In anoxic incubations with the addition of nitrite, approximately 65% of the nitrogen gas formation was due to anaerobic ammonium oxidation with nitrite, with the remainder being produced by denitrification. Anaerobic ammonium oxidation with nitrite exhibited a biological temperature response, with a rate optimum at 15degreesC and a maximum temperature of 37degreesC. The biological nature of the process and a 1:1 stoichiometry for the reaction between nitrite and ammonium indicated that the transformations might be attributed to the anammox process. Attempts to find other anaerobic ammonium-oxidizing processes in this sediment failed. The apparent K of nitrite consumption was less than 3 muM, and the relative importance of ammonium oxidation with nitrite and denitrification for the production of nitrogen gas was independent of nitrite concentration. Thus, the quantitative importance of ammonium oxidation with nitrite in the jar incubations at elevated nitrite concentrations probably represents the in situ situation. With the addition of nitrate, the production of nitrite from nitrate was four times faster than its consumption and therefore did not limit the rate of ammonium oxidation. Accordingly, the rate of this process was the same whether nitrate or nitrite was added as electron acceptor. The addition of organic matter did not stimulate denitrification, possibly because it was outcompeted by manganese reduction or because transport limitation was removed due to homogenization of the sediment.