Control of the contaminant level for determination of Al3+ using 8-quinolinol by high-performance liquid chromatography with fluorescence detection.
Control of the contaminant level for determination of Al3+ using 8-quinolinol by high-performance liquid chromatography with fluorescence detection.
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DOI：
10.1016/j.chroma.2008.03.018
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发表时间：
2008-05
期刊：
影响因子：
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通讯作者：
S. Saito;Junji Tamura;Makoto Sato;M. Aoyama
中科院分区：
文献类型：
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作者：
S. Saito;Junji Tamura;Makoto Sato;M. Aoyama
我们开发了一种在HPLC柱前衍生化步骤中使用动力学和热力学相结合的技术来控制污染物Al3+水平。该技术涉及用Al3+修饰传统的8-喹啉醇（Ox）配合物的高效液相色谱。在Ox和pH缓冲溶液中加入污染物抑制剂二羟基偶氮苯（DHAB）和1,2,3-三羟基苯（邻苯三酚），将这些溶液中的污染物Al3+转化为无活性配合物。样品中的Al3+选择性形成Ox配合物，反应速度快。然后在高效液相色谱中分离亚稳态的标记荧光配合物。这项技术成功地将污染抑制了17倍。该方法提高了检测限，并提供了稳定的空白。
We developed a technique controlling contaminant Al3+level using a combination of kinetics and thermodynamics at a pre-column derivatizing step in HPLC. The technique involves modifying a conventional HPLC of 8-quinolinol (Ox) complex with Al3+. The contaminant suppressing reagents, dihydroxyazobenzene (DHAB) and 1,2,3-trihydroxybenzene (pyrogallol) were added to the Ox and pH buffer solutions to convert contaminant Al3+in these solutions to inactive complexes. The Al3+in the sample selectively formed Ox complexes with fast kinetics. After that, the labeled fluorescent complexes in the resultant metastable state were separated in the HPLC. This technique successfully suppressed contamination by a factor of 17. This method allowed for an improvement in the detection limits and also provided a stable blank.