Changes in the distribution of laminin-5 during peri-implant epithelium formation after immediate titanium implantation in rats
Changes in the distribution of laminin-5 during peri-implant epithelium formation after immediate titanium implantation in rats
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DOI：
10.1016/j.biomaterials.2004.05.033
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Tanaka, T
中科院分区：
文献类型：
--
作者：
Atsuta, I;Yamaza, T;Tanaka, T
层粘连蛋白-5（Laminin-5，Ln-5）是基底膜的一种成分，调节上皮细胞的迁移和粘附。本研究采用抗Ln-5（γ 2链）抗体，观察了Ln-5在大鼠种植体周围上皮形成过程中的分布，并与拔牙后口腔黏膜形成过程中的分布进行了比较。一天后撤离。结合上皮（JE）消失。3天后，新的上皮从口腔沟上皮（OSE）形成，并在伤口上水平延伸，前沿有LN-5阳性细胞。5天后，从伤口两侧的OSE伸出的上皮连接并形成额外的新上皮。新的上皮在BM中表达Ln-5。1-2周后，从伤口两侧延伸的口腔上皮（OE）在中心连接。之后。OSE和新生上皮消失，仅OE保留覆盖伤口。植入（钛）后3天，无JE残留。新的上皮形成角化的OSE延伸与Ln-5阳性细胞的顶部。1-2周后。新的上皮变成PIE并朝向植入物表面进一步向顶端扩散。Ln-5在PIE-结缔组织界面表达。但没有（在植入物-PIE界面处）。最后，4周后，Ln-5在种植体-PIE界面表达。PIE为非角化上皮。这些发现表明Ln-5诱导细胞迁移和PIE形成。而这个PIE源自于OSE。此外，它们支持Ln-5有助于PIE 10钛附着的假设。而不管Ln-5在钛-PIE界面处的合成和沉积的延迟。(C)2004 Elsevier Ltd.保留所有权利。
Laminin-5 (Ln-5), a component of the basement membrane (BM), regulates epithetial cell migration and adhesion. This study used anti-Ln-5 (gamma2chain) antibody to investigate the distribution of Ln-5 during the formation of peri-implant epithelium (PIE) in rats, and compared it to the distribution of Ln-5 during oral mucosa formation after tooth extraction. One day after extraction. the junctional epithelium (JE) had disappeared. After 3 days, new epithelium formed from the oral sulcular epithelium (OSE) and extended horizontally over the wound with Ln-5-positive cells at the leading edge. After 5 days, the epithelium exiending from the OSE on each side of the wound joined and formed additional new epithelium. The new epithelium expressed Ln-5 in the BM - After 1-2 weeks, the oral epithelium (OE) extending from the sides of the wound joined in the center. Thereafter. OSE and new epithelium disappeared, and only OE remained covering the wound. Three days after implantation (titanium), no JE remained. New epithehium formed from the keratinized OSE extending apically with Ln-5-positive cells. After 1-2 weeks. the new epithelium became the PIE and spread further apically facing the implant surface. Ln-5 was expressed at the PIE-connective tissue interface. but no( at the implant-PIE interface. Finally, after 4 weeks, Ln-5 was expressed at the implant-PIE interface. and the PIE was non-keratinized epithelium. These findings suggest that Ln-5 induces cell migration clurinq PIE formation. and that PIE originates from OSE. Furthermore, they support the hypothesis that Ln-5 contributes to the attachment of PIE 10 titanium. regardless of the delay in the synthesis and deposition of Ln-5 at the titanium-PIE interface. (C) 2004 Elsevier Ltd. All rights reserved.