Structural Analysis of the Capsular Polysaccharide from Acinetobacter lwoffii F78
Structural Analysis of the Capsular Polysaccharide from Acinetobacter lwoffii F78
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DOI：
10.1002/ejoc.200800887
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发表时间：
2008-12-01
影响因子：
2.8
通讯作者：
Holst, Otto
中科院分区：
文献类型：
--
作者：
Hanuszkiewicz, Anna;Kaczynski, Zhigniew;Holst, Otto
从鲁氏不动杆菌F78菌株中分离纯化了荚膜多糖，并通过化学分析、核磁共振谱和质谱鉴定了其结构。通过透射电子显微镜实验证实了这种细菌上存在胶囊，并且利用抗脂质A单克隆抗体证明了分离材料的非内毒素来源。该结构代表了具有高度不均匀性的氨基取代基的新型非支化氨基多糖：-> 3）-α-L-FucNAc-（1 -> 3）-β-D-QuiNR（1）4NR（2）-（1 -> 4）-β-L-GlcNR（3）3NR（4）A-其中R-I = 3-羟基丁酸（3-HBA）或N-乙酰化丙氨酸（AlaNAc），R-2 = AlaNAc或3-HBA，R-3 =乙酰基或3-HBA，并且R-4 = 3-HBA或乙酰基。((C)Wiley-VCH Verlag GmbH & Co. KGaA，69451魏因海姆，德国，2008）
The capsular polysaccharide from Acinetobacter lwoffii F78 was isolated and purified, and its structure was elucidated by chemical analyses, NMR spectroscopy, and mass spectrometry. The presence of a capsule on this bacterium was confirmed by transmission electron microscopy experiments, and the utilization of the antilipid A monoclonal antibody proved the nonendotoxin origin of the isolated material. The structure represents a novel nonbranched aminopolysaccharide with high heterogeneity of amino groups substituents: -> 3)-alpha-L-FucNAc-(1 -> 3)-beta-D-QuiNR(1)4NR(2)-(1 -> 4)-beta-L-GlcNR(3)3NR(4)A-(1 ->, where R-1 = 3-hydroxybutyric acid (3-HBA) or N-acelylated alanine (AlaNAc), R-2 = AlaNAc or 3-HBA, R-3 = acetyl or 3-HBA, and R-4 = 3-HBA or acetyl. ((C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)