Spatial Distribution of Fungal Communities in an Arable Soil.
Spatial Distribution of Fungal Communities in an Arable Soil.
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DOI：
10.1371/journal.pone.0148130
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Krüger D
中科院分区：
文献类型：
--
作者：
Moll J;Hoppe B;König S;Wubet T;Buscot F;Krüger D
真菌是土壤生态过程的主要驱动力，因此不同土壤生态系统中的真菌群落已经得到了很好的研究。然而，对于耕地土壤分类解决细尺度的研究，包括真菌群落的垂直逐项仍然失踪。在这里，我们结合了一个克隆/桑格测序方法的ITS/LSU区域作为标记的一般真菌和部分SSU区域的丛枝菌根真菌（AMF）的微生物组在不同的玉米土壤生境的特点。在2009年和2010年的两个年度周期内分析了四个分区：a）0-10 cm的犁耕土壤，B）40-50 cm的生根土壤，c）60-70 cm土壤深度的无根土壤和d）玉米根。子囊菌门是所有隔室中最占优势的门。真菌群落，包括酵母菌和AMF之间的车厢差异很大。除其他外，Tetracladium，整体最大的MOTU（分子操作分类单位），发生在所有的车厢，而丝孢酵母菌占主导地位的所有土壤车厢。属于未分类的Helotiales序列形成最丰富的MOTU只存在于根中。这项研究为真菌的空间分布提供了新的见解，并有助于将真菌群落与特定的生态特性联系起来，例如不同的资源，这些资源是异质土壤环境中特定生态位的特征。
Fungi are prominent drivers of ecological processes in soils, so that fungal communities across different soil ecosystems have been well investigated. However, for arable soils taxonomically resolved fine-scale studies including vertical itemization of fungal communities are still missing. Here, we combined a cloning/Sanger sequencing approach of the ITS/LSU region as marker for general fungi and of the partial SSU region for arbuscular mycorrhizal fungi (AMF) to characterize the microbiome in different maize soil habitats. Four compartments were analyzed over two annual cycles 2009 and 2010: a) ploughed soil in 0–10 cm, b) rooted soil in 40–50 cm, c) root-free soil in 60–70 cm soil depth and d) maize roots. Ascomycota was the most dominant phylum across all compartments. Fungal communities including yeasts and AMF differed strongly between compartments. Inter alia, Tetracladium, the overall largest MOTU (molecular operational taxonomic unit), occurred in all compartments, whereas Trichosporon dominated all soil compartments. Sequences belonging to unclassified Helotiales were forming the most abundant MOTUs exclusively present in roots. This study gives new insights on spatial distribution of fungi and helps to link fungal communities to specific ecological properties such as varying resources, which characterize particular niches of the heterogeneous soil environment.