A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium)
A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium)
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DOI：
10.1534/genetics.166.1.389
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Paterson, AH
中科院分区：
文献类型：
--
作者：
Rong, JK;Abbey, C;Paterson, AH
我们报告了二倍体（D）和四倍体（AtDt）棉基因组的序列标记位点（STS），促进结构，功能和进化的基因组研究的遗传图谱。该图谱包括2584个1.72 cM（近似600 kb）的位点（AtDt）和763个1.96 cM（近似500 B）的位点（D）。Botl）二倍体和四倍体棉花表现出负交叉干扰，双重组体出乎意料地丰富。我们发现Dt和D染色体之间没有重大的结构变化，但证实了两个相互易位在染色体和几个倒位。在不同基因组的相应区域中的探针浓度可以代表着丝粒，而基因组特异性浓度可以代表异染色质。基因座复制模式揭示了所有13个预期的同源染色体组，并为更古老的多倍化事件可能早于600万至1100万年前的A-D分歧的可能性提供了新的支持。在312个RFLP序列中鉴定SSR，加上124个SSR的直接作图，以及CAPS和SNP的探索，说明了这些STS基因座在群体和检测系统中的“可移植性”，这些检测系统可用于标记辅助改良世界领先的纤维作物。这些数据为多倍体进化提供了新的见解，并为棉花基因组完整序列的组装奠定了基础。
We report genetic maps for diploid (D) and tetraploid (AtDt) Gossypium genomes composed of sequence-tagged sites (STS) that foster structural, functional, and evolutionary genomic studies. The maps include, respectively, 2584 loci at 1.72-cM (similar to600 kb) intervals based on 2007 probes (AtDt) and 763 loci at 1.96-cM (similar to500 b) intervals detected by 662 probes (D). Botl) diploid and tetraploid cottons exhibit negative crossover interference i.e., double recombinants are unexpectedly abundant. We found no major structural changes between Dt and D chromosomes, but confirmed two reciprocal translocations between At chromosomes and several inversions. Concentrations of probes in corresponding regions of the various genomes may represent centromeres, while genome-specific concentrations may represent heterochromatin. Locus duplication patterns reveal all 13 expected homeologous chromosomes sets and lend new support to the possibility that a more ancient polyploidization event may have predated the A-D divergence of 6-11 million years ago. Identification of SSRs within 312 RFLP sequences plus direct mapping of 124 SSRs and exploration for CAPS and SNPs illustrate Ihe "portability" of these STS loci across populations and detection systems useful for marker-assisted improvement of the world's leading fiber crop. These data provide new insights into polyploid evolution and represent a foundation for assembly of a finished sequence of the cotton genome.