The development of cell-adhesive hydrogel for 3D printing
The development of cell-adhesive hydrogel for 3D printing
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DOI：
10.18063/ijb.2016.02.002
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发表时间：
2016-01-01
影响因子：
8.4
通讯作者：
Nakamura, Makoto
中科院分区：
文献类型：
--
作者：
Arai, Kenichi;Tsukamoto, Yoshinari;Nakamura, Makoto
生物制造在制造功能性器官或组织方面引起了极大的关注。为了制造功能器官或组织，有必要再现组织特异性的微观到宏观结构。此前，我们开发了一款定制的 3D 生物打印机，能够打印和制造由活细胞组成的 3D 复杂水凝胶结构。通过凝胶化反应，可以通过逐层打印来制造精细且复杂的3D凝胶结构。海藻酸盐水凝胶的使用主要是由于其良好的制造性能。然而，由于其细胞粘附性不足，它不是组织再生的可靠平台。因此，我们的实验室有兴趣探索更适合生物打印和3D组织制造的水凝胶。在这项研究中，我们尝试制造具有足够细胞粘附特性的 3D 凝胶结构。我们专注于通过酶促反应形成水凝胶，通过结合带有酚羟基部分和辣根过氧化物酶的材料。我们检查了 Alg-Ph 和 Alg-Ph/Gelatin-Ph 凝胶。我们使用应用材料的混合溶液作为生物墨水并打印成H2O2溶液。我们成功制造了包括成纤维细胞培养物的 3D 凝胶片结构。在 3D 凝胶片中还观察到成纤维细胞增殖和活力超过一周。总之，通过酶反应获得的水凝胶是一种生物相容性生物墨水材料，可用于使用3D生物打印机制造3D细胞粘附凝胶结构。
Biofabrication has gained tremendous attention for manufacturing functional organs or tissues. To fabricate functional organs or tissues, it is necessary to reproduce tissue-specific micro to macro structures. Previously, we developed a custom-made 3D-bioprinter with the capability to print and fabricate 3D complicated hydrogel structures composed of living cells. Through the gelation reaction, fine and complicated 3D gel structures can be fabricated via layer by layer printing. Alginate hydrogel has been used mainly due to its good fabricating properties. However, it is not a reliable platform for tissue regeneration because of its inadequate cell-adhesiveness. Therefore, our laboratory is interested to explore more suitable hydrogels for bioprinting and 3D tissue fabrication. In this study, we tried to fabricate 3D gel structures with enough cell-adhesive properties. We focused on hydrogel formation through enzymatic reaction by incorporating materials bearing phenolic hydroxyl moieties and horseradish peroxidase. We examined Alg-Ph and Alg-Ph/Gelatin-Ph gels. We used a mixed solution of applied materials as bioink and printed into H2O2 solution. We successfully fabricated the 3D gel sheet structures including fibroblasts cultures. Fibroblast proliferation and viability were also observed in the 3D gel sheet for more than one week. In conclusion, the hydrogel obtained through enzymatic reaction is a biocompatible bioink material which can be applied to fabricate 3D cell-adhesive gel structures using a 3D-bioprinter.