In-depth proteomics and natural peptidomics analyses reveal antibacterial peptides in human endometrial fluid
In-depth proteomics and natural peptidomics analyses reveal antibacterial peptides in human endometrial fluid
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DOI：
10.1016/j.jprot.2020.103652
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发表时间：
2020-03-30
影响因子：
3.3
通讯作者：
Elortza, Felix
中科院分区：
文献类型：
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作者：
Azkargorta, Mikel;Bregon-Villahoz, Marta;Elortza, Felix
子宫内膜液的成分反映了子宫内膜的状态;它是胚胎着床过程和可能的病理状况的良好无创信息来源。虽然已经进行了一些尝试，其蛋白质组，其蛋白质的目录仍然不完整，并已做了很少的分析，它包含的天然肽。在这里，我们提出了一个全面的分析的蛋白质和天然肽的子宫内膜液。使用新型timsTOF Pro质谱仪分析了11名个体样本的蛋白质含量。我们鉴定了4694种蛋白质，其中至少一种肽的FDR <1%，其中2261种在> 50%的样品中发现。合并的子宫内膜液样品用于分离和分析天然肽。质谱分析从238种不同的蛋白质中鉴定出3899种天然存在的肽。其中，有一些推定的天然抗菌肽。使用微生物测定法证实了来自弹力素和Cu/Zn超氧化物歧化酶的肽的抗微生物活性。我们的研究结果大大扩展了已知的子宫内膜液蛋白质的目录，并提供了广泛的新信息，这种fluid.Significance的天然肽含量：子宫内膜液中含有许多蛋白质，其临床意义仍然是未知的。有些可能只是子宫内膜功能的标志物，但其他可能在胚胎营养和/或植入中发挥作用。人类子宫内膜液分析可能为体外受精项目中胚胎移植策略的新发展打开大门，并导致胚胎培养基成分的改进。在这里，我们报告，第一次，子宫内膜液肽的抗菌活性。这些肽可能在最近描述的子宫微生物群的平衡中发挥重要作用。
The composition of endometrial fluid reflects the status of the endometrium; it is a good atraumatic source of information on embryo implantation processes and possible pathological conditions. Although some attempts have been made to characterise its proteome, the catalogue of its proteins remains incomplete and little has been done to analyse the natural peptides it contains. Here, we present a comprehensive analysis of the proteins and natural peptides of the endometrial fluid. The protein content of samples from 11 individuals was analysed using the novel timsTOF Pro mass spectrometer. We identified 4694 proteins with at least one peptide with FDR < 1%, of which 2261 were found in > 50% of the samples. A pooled endometrial fluid sample was used for isolation and analysis of the natural peptides. Mass spectrometry analysis identified 3899 naturally occurring peptides from 238 different proteins. Among these, there were some putative natural antibacterial peptides. Antimicrobial activity of peptides derived from elafin and Cu/Zn superoxide dismutase was confirmed using microbiological assays. Our results substantially expand the catalogue of known endometrial fluid proteins and provide extensive new information on the natural peptide content of this fluid.Significance: The endometrial fluid contains many proteins whose clinical relevance is still unknown. Some might be merely markers of endometrial function, but others might play a role in embryo nutrition and/or implantation. Human endometrial fluid analysis might open the door to new developments in embryo transfer strategies in in-vitro fertilisation programmes and lead to improvements in the composition of embryo culture media. Here, we report, for the first time, antimicrobial activity of endometrial fluid peptides. Such peptides could play an important role in the balance of the recently described uterine microbiota.