Contrasting relationships between the diversity of candidate genes and variation of bud burst in natural and segregating populations of European oaks
Contrasting relationships between the diversity of candidate genes and variation of bud burst in natural and segregating populations of European oaks
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DOI：
10.1038/hdy.2009.134
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发表时间：
2010-05-01
期刊：
影响因子：
3.8
通讯作者：
Kremer, A.
中科院分区：
文献类型：
--
作者：
Derory, J.;Scotti-Saintagne, C.;Kremer, A.
在9个候选基因（CGs）（共4.6 kb）内的核苷酸多样性进行了评估的时间在9个无柄橡树（栎petraea）分布在欧洲中部和北方人口的萌芽。取样群体的选择是基于其在普通花园实验（种源试验）中观察到的萌芽时间的对比。根据其在从静止到发育芽的过渡期间的表达谱和/或其在模型植物中的功能作用来选择CG。总体核苷酸多样性很大（pi（tot）= 6.15 x 10（-3）; pi（silent）= 11.2 x 10（-3）），但群体分化并不大于微卫星。在125个单核苷酸多态性（SNP）中没有发现偏离中性预期的离群SNP。这些结果与在分离群体中观察到的CG和萌芽之间的显著关联形成鲜明对比。通过13年的定位季节观察，对一个克隆定位系谱的萌芽数量性状位点（QTL）进行了定位。共检测到19个QTL，位于连锁群2、5、9上的QTL对该性状表型变异的贡献率均在12%以上。8个基因在该家系的双亲中具有多态性，可以在现有的遗传图谱上定位。其中5个位于萌芽QTL的置信区间内。有趣的是，其中四个位于三个QTL表现出最大的贡献萌芽。Heredity（2010）104，438-448; doi：10.1038/hdy.2009.134; 2009年10月7日在线发表
Nucleotide diversity was assessed within nine candidate genes (CGs) (in total 4.6 kb) for the time of bud burst in nine sessile oak (Quercus petraea) populations distributed in central and northern Europe. The sampled populations were selected on the basis of their contrasting times of bud burst observed in common garden experiments (provenance tests). The CGs were selected according to their expression profiles during the transition from quiescent to developing buds and/or their functional role in model plants. The overall nucleotide diversity was large (pi(tot) = 6.15 x 10(-3); pi(silent) = 11.2 x 10(-3)), but population differentiation was not larger than for microsatellites. No outlier single-nucleotide polymorphism (SNP) departing from neutral expectation was found among the total of 125 SNPs. These results contrasted markedly with the significant associations that were observed between the CGs and bud burst in segregating populations. Quantitative trait loci (QTLs) for bud burst were identified for 13 year*site seasonal observations in a cloned mapping pedigree. Nineteen QTLs were detected, and QTLs located on linkage groups 2, 5 and 9 contributed repeatedly to more than 12% of the phenotypic variation of the trait. Eight genes were polymorphic in the two parents of the pedigree and could be mapped on the existing genetic map. Five of them located within the confidence intervals of QTLs for bud burst. Interestingly, four of them located within the three QTLs exhibiting the largest contributions to bud burst. Heredity (2010) 104, 438-448; doi:10.1038/hdy.2009.134; published online 7 October 2009