Genetic diversity of the endangered species Rosa rugosa Thunb. in China and implications for conservation strategies
Genetic diversity of the endangered species Rosa rugosa Thunb. in China and implications for conservation strategies
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DOI：
10.1111/j.1759-6831.2009.00049.x
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发表时间：
2009-11
影响因子：
3.7
通讯作者：
Ji-hong Yang;Shuping Zhang;Jian Liu;Wenji Zhai;Ren-qing Wang
中科院分区：
文献类型：
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作者：
Ji-hong Yang;Shuping Zhang;Jian Liu;Wenji Zhai;Ren-qing Wang
摘要玫瑰是我国北方河口砂丘和卵石滩的优势种和重要灌木树种之一。然而，在过去的二十年里，由于栖息地的退化和丧失，其分布面积、种群数量和每个种群的大小都迅速减少。利用随机扩增多态性DNA标记技术，分析了四个大种群的遗传多样性。并探讨了该濒危物种在我国的有效保护策略。高的遗传变异检测R。中国的rugosa种群。4个地方群体的多态位点百分率（P%）平均为57.99%，占总群体的P%为75.30%。平均香农信息指数（H 0）为0.2826，而总H 0为0.3513。居群间的遗传分化为0.1878，表明居群内的遗传多样性最大。图们江群体的遗传多样性最高（P%= 66.27%，H 0 = 0.3117），有2条特异带。长山岛居群具有较高的遗传多样性（P%= 59.04%，H 0 = 0.3065）。TMJ和CSD群体中的中高频位点分别占总群体的95.33%和99.33%（P>0.05）。这些结果表明，居群TMJ和CSD应优先考虑就地保护，并作为迁地保护的种子或繁殖体来源。本研究的结果还表明，R。中国的Rugosa濒危是由于人类活动的结果，而不是种群的遗传衰退，因此，在Rugosa的保护中应绝对禁止人为干扰。rugosa生境。
Abstract Rosa rugosa Thunb. is one of the dominant and important shrub species in estuary dunes and shingle beaches of northern China. However, its area of distribution, the number of populations, and the size of each population have decreased rapidly in the past two decades because of habitat degradation and loss. Random amplified polymorphic DNA markers were used to determine the genetic diversity of four remaining large natural populations of R. rugosa and to discuss an effective conservation strategy for this endangered species in China. High genetic variations were detected in R. rugosa populations in China. The mean percentage of polymorphic loci (P%) within four local populations was 57.99%, with the P% of the total population being 75.30%. Mean Shannon's information index (H0) was 0.2826, whereas total H0 was 0.3513. The genetic differentiation among populations was 0.1878, which indicates that most genetic diversity occurs within populations. Population Tumenjiang (TMJ) showed the highest genetic diversity (P%= 66.27%; H0= 0.3117) and contained two exclusive bands. Population Changshandao (CSD) showed higher genetic diversity (P%= 59.04%; H0= 0.3065). Populations TMJ and CSD contained 95.33% and 99.33%, respectively, of loci with moderate to high frequency (P>0.05) of the total population. These results indicate that populations TMJ and CSD should be given priority for in situ conservation and regarded as seed or propagule sources for ex situ conservation. The results of the present study also suggest that R. rugosa in China has become endangered as a result of human actions rather than genetic depression of populations; thus, human interference should be absolutely forbidden in R. rugosa habitats.