Determination of carotenoids in Dunaliella salina cultivated in Taiwan and antioxidant capacity of the algal carotenoid extract
Determination of carotenoids in Dunaliella salina cultivated in Taiwan and antioxidant capacity of the algal carotenoid extract
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DOI：
10.1016/j.foodchem.2007.12.043
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发表时间：
2008-07-15
期刊：
影响因子：
8.8
通讯作者：
Yang, Deng-Jye
中科院分区：
文献类型：
--
作者：
Hu, Chao-Chin;Lin, Jau-Tien;Yang, Deng-Jye
建立了一种简单、分离效果良好的高效液相色谱法测定台湾盐藻中全反式和顺式类胡萝卜素的含量。分析使用C30柱（250 x 4.6 mm，5 μ m）和混合甲醇-乙腈-水（84/14/2，v/v/v）和二氯甲烷（75/25，v/v）的等度溶剂系统（流速= 1 mL/min）。在450 nm处检测类胡萝卜素。此外，还通过Trolox等效抗氧化能力（TEAC）测定、还原能力和2，2-二苯基-2-苦肼基水合物（DPPH）自由基清除能力测定来评价藻类胡萝卜素提取物的抗氧化能力。结果表明，在30 min内，藻液中7种类胡萝卜素可同时分离，总量为290.77 mg/ g藻。藻体中主要类胡萝卜素全反式β-胡萝卜素和9-或9 '-顺式β-胡萝卜素的含量分别为138.25和124.65 mg/g藻。全反式叶黄素、全反式玉米黄质、13-或13 '-顺式-β-胡萝卜素、全反式-α-胡萝卜素和9-或9'-顺式-α-胡萝卜素的含量分别为6.55、11.27、4.95、2.69和2.41 mg/g藻。藻类类胡萝卜素提取物具有显着更高的抗氧化活性比全反式形式的α-胡萝卜素，β-胡萝卜素，叶黄素和玉米黄质在所有的抗氧化剂测定。类胡萝卜素的顺式形式，特别是9-或9 '-顺式-β-胡萝卜素，可能对藻类提取物的抗氧化能力起关键作用。(C)2008爱思唯尔有限公司保留所有权利。
A simple HPLC method with good separation efficiency was developed to determine all-trans and cis forms of carotenoids in Dunaliella salina cultivated in Taiwan. The analysis used a C30 column (250 x 4.6 mm, 5 mu m) and an isocratic solvent system (flow rate = 1 mL/min) mixing methanol-acetonitrile-water (84/14/2, v/v/v) and methylene chloride, (75/25, v/v). Carotenoids were detected at 450 nm. Moreover, the antioxidant capacities of the algal carotenoid extract were also evaluated with Trolox equivalent antioxidant capacity (TEAC) assay, reducing power and 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) radical scavenging assay. Results showed that 7 carotenoids in the algal extract could be separated simultaneously within 30 min and the total amount of them was 290.77 mg/ g algae. The contents of all-trans-beta-carotene and 9- or 9'-cis-beta-carotene, the major carotenoids in the algae, were 138.25 and 124.65 mg/g algae, respectively. The contents of all-trans-lutein, all-trans-zeaxanthin, 13- or 13'-cis-beta-carotene, all-trans-alpha-carotene and 9- or 9'-cis-alpha-carotene were 6.55, 11.27, 4.95, 2.69, and 2.41 mg/g algae, respectively. The algal carotenoid extract had significantly higher antioxidant activity than all-trans forms of alpha-carotene, beta-carotene, lutein and zeaxanthin in all antioxidant assays. The cis forms of carotenoids, especially 9- or 9'-cis-beta-carotene, might play crucial roles for the antioxidant capacities of the algal extract. (C) 2008 Elsevier Ltd. All rights reserved.