Posttranslational degradation of the major myelin glycoprotein by Schwann cells in vivo and in vitro.
Posttranslational degradation of the major myelin glycoprotein by Schwann cells in vivo and in vitro.
复制标题
体内和体外雪旺细胞对主要髓磷脂糖蛋白的翻译后降解。
DOI：
10.1111/j.1749-6632.1990.tb42395.x
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发表时间：
1990
影响因子：
5.2
通讯作者：
Poduslo,JF
中科院分区：
文献类型：
--
作者：
Brunden,KR;Poduslo,JF
周围神经髓鞘的主要糖蛋白Po的表达在两种体内实验范例中进行了研究：成年大鼠坐骨神经的撞击损伤后和永久性横断损伤后。这些模型最初的特征是沃勒变性，随后分别出现或不出现随后的轴突再生和髓鞘再生。已经证明，与挤压损伤的神经相比，新合成的Pol4及其mRNA5+的水平在横断损伤后降低，并且其翻译后寡糖加工改变。“使用体内永久性横断范例，”我们已经表明，在横断神经中PO的一些明显下调是糖蛋白在其生物合成后不久溶酶体降解的结果。这种降解可通过溶酶体扰动或改变寡糖加工程度来抑制。因此，蛋白catalysts，似乎是一种翻译后机制，用于调节水平的Po表达的髓鞘形成的情况下。下一章进一步解决了这种调节在体内的永久性横断神经，并比较它与表达的调节，发生在体外培养后，从新生儿和年轻的神经内膜外植体的雪旺细胞。
The expression of the major glycoprotein of peripheral nerve myelin, Po, has been investigated in two in viuo experimental paradigms: after crash injury of the sciatic nerve of the adult rat and after permanent transection injury.'-3 These models are initially characterized by Wallerian degeneration, followed by the presence or absence of subsequent axonal regeneration and remyelination, respectively. It has been demonstrated that the level of newly synthesized Pol4 and its mRNA5*" is reduced after transection injury and its posttranslational oligosaccharide processing is altered when compared to crush-injured nerve..'Using the in viw permanent transection paradigm,'we have shown that some of the apparent down-regulation of PO in the transected nerve is the result of lysosomal degradation of the glycoprotein shortly after its biosynthesis. This degradation is inhibited by lysosomal perturbation or by altering the degree of oligosaccharide processing. Protein catabolism, therefore, appears to be a posttranslational mechanism for regulating the level of Po expression in the absence of myelination. The following chapter further addresses this regulation in the permanently transected nerve in vivo and compares it with the regulation of expression that occurs in vitro after culturing Schwann cells from neonatal and young endoneurial explants.