Nitrogen source and the retrograde signalling pathway affect detection, not generation, of the [URE3] prion
Nitrogen source and the retrograde signalling pathway affect detection, not generation, of the [URE3] prion
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DOI：
10.1002/yea.1398
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发表时间：
2006-08-01
期刊：
影响因子：
2.6
通讯作者：
Wickner, Reed B.
中科院分区：
文献类型：
--
作者：
Edskes, Herman K.;Naglieri, Benedetta M.;Wickner, Reed B.
[URE3]是Ure2p的感染性（朊病毒）非活性淀粉样蛋白形式，Ure2p是氮催化剂的调节剂。在NH4+上选择[URE3]克隆，使用其DAL 5的去抑制表达以允许脲基琥珀酸酯的摄取（USA）。我们以前报道过mks1 Delta可以阻止[URE3]的产生，其他人报道过培养基中的谷氨酸或mks1 Delta菌株中升高的戊二酸可以阻断[URE3]的产生。我们在这里表明，谷氨酸盐的升高不会阻止[URE3]的产生，但mks1 Delta也不会。相反，通过逆行调节途径对mks] A的DAL 5的转录后效应阻止检测含有[URE3]朊病毒的集落。此外，氨和谷氨酸盐的存在阻止USA吸收。已知的[URE3]菌株，因此朊病毒的检测被阻止，而不是其产生。版权所有（c）2006约翰·米利利父子有限公司
[URE3] is an infectious (prion) inactive amyloid form of Ure2p, a regulator of nitrogen catabolism. [URE3] clones are selected on NH4+, using their derepressed expression of DAL5 to allow uptake of ureidosuccinate (USA). We previously reported that mks1 Delta prevents generation of [URE3] and others reported that glutamate in the medium or the elevated glutarnate in mks1 Delta strains blocks [URE3] generation. We show here that elevated glutamate does not block [URE3] generation, but that neither does mks1 Delta. Rather, a post-transcriptional effect on DAL5 of mks] A through the retrograde regulation pathway prevents detection of [URE3] prion-containing colonies. Moreover, the presence of both ammonia and glutamate blocks USA uptake. a known [URE3] strain, so that detection of the prion is prevented, rather than its generation. Copyright (c) 2006 John Mliley & Sons, Ltd.