Stem Cells in Dental Pulp of Deciduous Teeth
Stem Cells in Dental Pulp of Deciduous Teeth
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DOI：
10.1089/ten.teb.2011.0327
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发表时间：
2012-04-01
影响因子：
6.4
通讯作者：
Caplan, Arnold I.
中科院分区：
文献类型：
--
作者：
Kerkis, Irina;Caplan, Arnold I.
乳牙（乳牙）的牙髓在脱落后被丢弃，是年轻干细胞的有利来源。在此，我们讨论乳牙干细胞（DTSC）的分离和培养方法。我们表明，基于这些方法，至少可以鉴定出三种不同的干细胞群体：类似于骨髓来源的间充质干细胞的群体、上皮干细胞样细胞和/或由两种细胞类型组成的混合群体。我们分析了 DTSC 的胚胎起源和干细胞生态位，了解它们可以为其未来在细胞治疗和再生医学中的应用提供的优势。 DTSC群体的体外和体内分化、其发育潜力、免疫相容性、组织工程和动物模型研究中的移植应用也是本报告的重点。我们简要描述了从 DTSC 中衍生出诱导多能干 (iPS) 细胞，与人类成纤维细胞相比，可以更容易、更有效地获得诱导多能干 (iPS) 细胞。这些 iPS 细胞代表了研究儿科疾病和病症的有趣模型。还讨论了 DTSC 银行业务的重要性。
Dental pulp from deciduous (baby) teeth, which are discarded after exfoliation, represents an advantageous source of young stem cells. Herein, we discuss the methods of deciduous teeth stem cell (DTSC) isolation and cultivation. We show that based on these methods, at least three different stem cell populations can be identified: a population similar to bone marrow-derived mesenchymal stem cells, an epithelial stem-like cells, and/or a mixed population composed of both cell types. We analyzed the embryonic origin and stem cell niche of DTSCs with respect to the advantages they can provide for their future use in cell therapies and regenerative medicine. In vitro and in vivo differentiation of the DTSC populations, their developmental potential, immunological compatibility, tissue engineering, and transplantation use in studies in animal models are also the focus of the current report. We briefly describe the derivation of induced pluripotent stem (iPS) cells from DTSCs, which can be obtained more easily and efficiently in comparison with human fibroblasts. These iPS cells represent an interesting model for the investigation of pediatric diseases and disorders. The importance of DTSC banking is also discussed.