Genetic identification of avian hepatitis E virus (HEV) from healthy chicken flocks and characterization of the capsid gene of 14 avian HEV isolates from chickens with hepatitis-splenomegaly syndrome in different geographical regions of the United States
Genetic identification of avian hepatitis E virus (HEV) from healthy chicken flocks and characterization of the capsid gene of 14 avian HEV isolates from chickens with hepatitis-splenomegaly syndrome in different geographical regions of the United States
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DOI：
10.1099/vir.0.19582-0
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发表时间：
2004-03-01
影响因子：
3.8
通讯作者：
Meng, XJ
中科院分区：
文献类型：
--
作者：
Sun, ZF;Larsen, CT;Meng, XJ
禽戊型肝炎病毒（HEV）是从患有肝炎-脾细胞阴性（HS）综合征的鸡群中分离到的一种新病毒，与人戊型肝炎病毒（HEV）在遗传和抗原性上都有相似性。近年来，研究发现禽戊型肝炎病毒抗体在健康鸡群中也普遍存在。对一个已知血清学阳性但健康的养鸡场进行了前瞻性研究，以鉴定来自健康鸡的禽戊型肝炎病毒分离株。随机选择14只鸡，在自然条件下标记和监测19周。所有14只鸡在12周龄研究开始时均为血清阴性。到21周龄时，所有14只鸡均已血清转化为禽HEV抗体。没有鸡有任何症状的HS综合征。从健康鸡中分离的禽戊型肝炎病毒（HEV）解旋酶基因和衣壳蛋白基因的部分序列与从HS综合征鸡中分离的HEV的相应区域的核苷酸序列同源性为75- 97%。到目前为止，只有一个株禽戊型肝炎病毒从鸡与HIS综合征已被遗传特征的衣壳基因，因此，衣壳基因区的额外的14个分离物从鸡与HS综合征也进行了表征。从HS综合征鸡群中分离的禽戊型肝炎病毒衣壳蛋白基因具有异质性，核苷酸序列同源性为76- 100%。这项研究表明，禽戊型肝炎病毒是地方性的鸡群和亚临床传播的鸡在美国和病毒是异质性的。
Avian hepatitis E virus (HEV), a novel virus identified from chickens with hepatitis-splenornegaly (HS) syndrome, is genetically and antigenically related to human HEV. Recently, it was found that avian HEV antibody is also prevalent in healthy chickens. A prospective study was done on a known seropositive but healthy chicken farm to identify avian HEV isolates from healthy chickens. Fourteen chickens were randomly selected, tagged and monitored under natural conditions for 19 weeks. All 14 chickens were seronegative at the beginning of the study at 12 weeks of age. By 21 weeks of age, all 14 chickens had seroconverted to avian HEV antibody. None of the chickens had any sign of HS syndrome. Partial helicase gene and capsid gene sequences of avian HEV isolates recovered from a healthy chicken were determined and found to share 75-97 % nucleotide sequence identity with the corresponding regions of avian HEV isolates from chickens with HS syndrome. Thus far, only one strain of avian HEV from a chicken with HIS syndrome has been genetically characterized for its capsid gene, therefore the capsid gene region of an additional 14 isolates from chickens with HS syndrome were also characterized. The capsid genes of avian HEV isolates from chickens with HS syndrome were found to be heterogeneic, sharing 76-100 % nucleotide sequence identity with each other. This study indicates that avian HEV is enzootic in chicken flocks and spreads subclinically among chickens in the United States and that the virus is heterogeneic.