Processing and stability of inducibly expressed rpsO mRNA derivatives in Bacillus subtilis.
Processing and stability of inducibly expressed rpsO mRNA derivatives in Bacillus subtilis.
复制标题
枯草芽孢杆菌中诱导表达的 rpsO mRNA 衍生物的加工和稳定性。
DOI：
10.1128/jb.00740-09
复制
发表时间：
2009
影响因子：
3.2
通讯作者：
Bechhofer,DavidH
中科院分区：
文献类型：
--
作者：
Yao,Shiyi;Bechhofer,DavidH
TheBacillus subtilis rpsOgene specifies a small (388-nucleotide), monocistronic mRNA that encodes ribosomal protein S15. We showed earlier thatrpsOmRNA decay intermediates accumulated to a high level in a strain lacking polynucleotide phosphorylase. Here, we used inducibly expressed derivatives ofrpsO, encoding smaller RNAs that had the complex 5′ region deleted, to study aspects of mRNA processing inB. subtilis. An IPTG (isopropyl-β-d-thiogalactopyranoside)-induciblerpsOtranscript that containedlacsequences at the 5′ end, calledlac-rpsORNA, was shown to undergo processing to result in an RNA that was 24 nucleotides shorter than full length. Such processing was dependent on the presence of an accessible 5′ terminus; alac-rpsORNA that contained a strong stem-loop at the 5′ end was not processed and was extremely stable. Interestingly, this stability depended also on ribosome binding to a nearby Shine-Dalgarno sequence but was independent of downstream translation. Either RNase J1 or RNase J2 was capable of processinglac-rpsORNA, demonstrating for the first time a particular in vivo processing event that could be catalyzed by both enzymes. Decay intermediates were detected in thepnpAstrain only for alac-rpsORNA that was untranslated. Analysis of processing of an untranslatedlac-rpsORNA in thepnpAstrain shortly after induction of transcription suggested that endonuclease cleavage at 3′-proximal sites was an early step in turnover of mRNA.
