Ultrasensitive Label-Free Detection of PNA-DNA Hybridization by Reduced Graphene Oxide Field-Effect Transistor Biosensor
Ultrasensitive Label-Free Detection of PNA-DNA Hybridization by Reduced Graphene Oxide Field-Effect Transistor Biosensor
复制标题
通过还原氧化石墨烯场效应晶体管生物传感器对 PNA-DNA 杂交进行超灵敏无标记检测
DOI：
10.1021/nn4063424
复制
发表时间：
2014-03-01
期刊：
影响因子：
17.1
通讯作者：
Zhang, Guo-Jun
中科院分区：
文献类型：
--
作者：
Cai, Bingjie;Wang, Shuting;Zhang, Guo-Jun
报道了一种基于还原氧化石墨烯（R-GO）的场效应晶体管（FET）生物传感器，用于肽核酸（PNA）DNA杂交超灵敏无标记检测DNA。本工作通过肼还原氧化石墨烯制备了R-GO，并将其滴涂在传感器表面制备了FET生物传感器。采用PNA代替DNA作为捕获探针，并通过R-GO FET生物传感器通过PNA DNA杂交进行DNA检测。检测限低至100 fM，这是一个数量级低于先前报道的石墨烯FET DNA生物传感器的基础上DNA DNA杂交。此外，R-GO FET生物传感器能够区分互补DNA与单碱基错配DNA和非互补DNA。有趣的是，所制造的DNA生物传感器被发现具有再生能力。所开发的R-GO FET DNA生物传感器显示出超灵敏度和高特异性，表明其作为即时护理工具在疾病诊断中的潜在应用。
A reduced graphene oxide (R-GO)-based field-effect transistor (FET) biosensor used for ultrasensitive label-free detection of DNA via peptide nucleic acid (PNA) DNA hybridization is reported. In this work, R-GO was prepared by reduction of GO with hydrazine, and the FET biosensor was fabricated by drop-casting the R-GO suspension onto the sensor surface. PNA instead of DNA as the capture probe was employed, and DNA detection was performed through PNA DNA hybridization by the R-GO FET biosensor. The detection limit as low as 100 fM was achieved, which is 1 order of magnitude lower than that of the previously reported graphene FET DNA biosensor based on DNA DNA hybridization. Moreover, the R-GO FET biosensor was able to distinguish the complementary DNA from one-base mismatched DNA and noncomplementary DNA. Interestingly, the fabricated DNA biosensor was found to have a regeneration capability. The developed R-GO FET DNA biosensor shows ultrasensitivity and high specificity, indicating its potential applications in disease diagnostics as a point-of-care tool.