Simultaneous determination of soluble sugars and organic acids as their trimethylsilyl derivatives in apricot fruits by gas-liquid chromatography
Simultaneous determination of soluble sugars and organic acids as their trimethylsilyl derivatives in apricot fruits by gas-liquid chromatography
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DOI：
10.1016/s0021-9673(96)00709-1
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发表时间：
1997-01-10
影响因子：
4.1
通讯作者：
Cristoferi, G
中科院分区：
文献类型：
--
作者：
Bartolozzi, F;Bertazza, G;Cristoferi, G
本研究的目的是确定一个可靠的程序定量的有机酸，醇溶性糖和糖醇的果肉通过一个快速的GLC方法，而不诉诸于甲氧基化的糖，并采用杏作为模型。使用两个内部标准，准确的衍生化和适当的校准的GLC条件允许一个准确的定量分析的化合物中检测到的未知样品。这种简单的方法提高了三甲基甲硅烷基衍生物的制备速度，并且具有高度的重现性。年之间的变异性被发现的五个品种的研究和每个化合物的绝对值，而发病率的单糖作为一个整体的百分比是更稳定的两年的观察。
The aim of this study was to determine a reliable procedure for the quantification of organic acids, alcohol soluble sugars and sugar alcohols in fruit flesh by means of a rapid GLC method, without resorting to methoximation of sugars and employing apricot as a model. The use of two internal standards, an accurate derivatization and a proper calibration of the GLC conditions allowed an accurate quantitative analysis of the compounds detected in the unknown samples. This simple procedure improves the speed of preparation of the trimethylsilyl derivatives and is highly reproducible. Variability was found between years for each of the five cultivars studied and for each compound in terms of absolute values, whereas the percentage incidence of the single sugars as a total was more stable over the two years of observation.