Getting to Compliance in Forced Exercise in Rodents: A Critical Standard to Evaluate Exercise Impact in Aging-related Disorders and Disease
Getting to Compliance in Forced Exercise in Rodents: A Critical Standard to Evaluate Exercise Impact in Aging-related Disorders and Disease
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DOI：
10.3791/51827
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发表时间：
2014-08-01
影响因子：
1.2
通讯作者：
Salvatore, Michael F.
中科院分区：
文献类型：
--
作者：
Arnold, Jennifer C.;Salvatore, Michael F.
人们越来越认识到运动对改善具有神经生物学基础的几种疾病状态的积极影响;这些包括改善认知功能和身体表现。因此，采用运动的动物研究数量有所增加。有人认为，一个内在的价值，强迫运动是研究人员控制的因素，可以影响运动对行为结果的影响，特别是运动频率，持续时间和强度的运动方案。然而，强制运动方案的依从性可能是一个问题，特别是如果要避免采用足部电击的潜在混淆。同样重要的是要考虑到，由于大多数认知和运动障碍发生在老年个体中，因此确定运动对这些障碍的影响应考虑使用具有最高可能依从性水平的老年啮齿动物，以确保对测试受试者的需求最小。在这里，将介绍和讨论在老年啮齿动物模型中实现接近100%的跑步机运动依从性所需的相关步骤和注意事项。尽管研究者采用了特定的运动方案，但我们的方案应该对那些对强迫运动对衰老相关损伤（包括衰老相关帕金森综合征和帕金森病）的潜在影响特别感兴趣的研究者有用。
There is a major increase in the awareness of the positive impact of exercise on improving several disease states with neurobiological basis; these include improving cognitive function and physical performance. As a result, there is an increase in the number of animal studies employing exercise. It is argued that one intrinsic value of forced exercise is that the investigator has control over the factors that can influence the impact of exercise on behavioral outcomes, notably exercise frequency, duration, and intensity of the exercise regimen. However, compliance in forced exercise regimens may be an issue, particularly if potential confounds of employing foot-shock are to be avoided. It is also important to consider that since most cognitive and locomotor impairments strike in the aged individual, determining impact of exercise on these impairments should consider using aged rodents with a highest possible level of compliance to ensure minimal need for test subjects. Here, the pertinent steps and considerations necessary to achieve nearly 100% compliance to treadmill exercise in an aged rodent model will be presented and discussed. Notwithstanding the particular exercise regimen being employed by the investigator, our protocol should be of use to investigators that are particularly interested in the potential impact of forced exercise on aging-related impairments, including aging-related Parkinsonism and Parkinson's disease.