CsSAP, a teleost serum amyloid P component, interacts with bacteria, promotes phagocytosis, and enhances host resistance against bacterial and viral infection
CsSAP, a teleost serum amyloid P component, interacts with bacteria, promotes phagocytosis, and enhances host resistance against bacterial and viral infection
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DOI：
10.1016/j.dci.2015.10.002
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发表时间：
2016-02-01
影响因子：
2.9
通讯作者：
Sun, Li
中科院分区：
文献类型：
--
作者：
Wang, Ting;Sun, Li
血清淀粉样蛋白P组分(SAP)是五肽家族中的一员，在脊椎动物的天然免疫中起着重要作用。在鱼类中，SAP的生物学功能基本上是未知的。在这项研究中，我们研究了舌底半滑舌肌SAP同源物(CsSAP)的表达和功能。CsSAP与已知鱼类SAP的总序列同源性为46%-58%，并因细菌和病毒感染而上调表达。重组CsSAP(RCsSAP)与多种革兰氏阳性和革兰氏阴性细菌显示出不同的结合能力，并促进宿主吞噬细胞对结合细菌的摄取。当在体内导入rCsSAP时，不仅增强了宿主对细菌感染的抵抗力，而且还增强了对病毒感染的抵抗力。CsSAP的抗体封闭显著削弱了舌底清除入侵细菌的能力。这些结果提供了第一个证据，表明鱼汁液对抗菌和抗病毒免疫都有显著贡献。(C)2015爱思唯尔有限公司。保留所有权利。
Serum amyloid P component (SAP) is a member of the pentraxins family that plays important roles in innate immunity in vertebrates. In fish, the biological function of SAP is essentially unknown. In this study, we examined the expression and function of a SAP homologue (CsSAP) from tongue sole Cynoglossus semilaevis. CsSAP shares 46%-58% overall sequence identities with known fish SAP and was upregulated in expression by bacterial and viral infection. Recombinant CsSAP (rCsSAP) exhibited differential binding capacities to a wide range of Gram-positive and Gram-negative bacteria and promoted uptake of the bound bacteria by host phagocytes. When introduced in vivo, rCsSAP enhanced host resistance not only to bacterial infection but also to viral infection. Consistently, antibody blockage of CsSAP significantly weakened the ability of tongue sole to clear invading bacteria. These results provide the first evidence that fish SAP contributes significantly to both antibacterial and antiviral immunities. (C) 2015 Elsevier Ltd. All rights reserved.