Time-lapse cinematography of dynamic changes occurring during in vitro development of human embryos
Time-lapse cinematography of dynamic changes occurring during in vitro development of human embryos
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DOI：
10.1016/j.ajog.2008.07.023
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发表时间：
2008-01-01
影响因子：
9.8
通讯作者：
Maeda, Kazuo
中科院分区：
文献类型：
--
作者：
Mio, Yasuyuki;Maeda, Kazuo
目的：本研究的目的是通过使用延时电影摄影术（TLC）来阐明早期人类胚胎的发育变化。对于人类卵子，受精和卵裂，胚泡发育和孵化，以及随之而来的变化被重复拍摄，每隔5-用倒置显微镜在稳定的温度和pH值下观察6天，以30帧/秒的速度拍摄照片并研究电影。电影摄影增加了我们对人类早期胚胎受精、发育和行为的形态学机制的了解，并根据体外孵育时间延长至囊胚阶段确定了单卵双胎妊娠的风险增加。结论：利用薄层层析技术，我们观察了单个精子对卵子的受精，随后的早期卵裂，桑椹胚的形成，囊胚的孵化，胚胎板的变化，以及从孵化的囊胚发育成单卵双胞胎。
OBJECTIVE: The purpose of this study was to clarify developmental changes of early human embryos by using time-lapse cinematography (TLC).STUDY DESIGN: For human ova, fertilization and cleavage, development of the blastocyst, and hatching, as well as consequent changes were repeatedly photographed at intervals of 5-6 days by using an inverse microscope under stabilized temperature and pH. Photographs were taken at 30 frames per second and the movies were studied.RESULTS: Cinematography has increased our understanding of the morphologic mechanisms of fertilization, development, and behavior of early human embryos, and has identified the increased risk of monozygotic twin pregnancy based on prolonged incubation in vitro to the blastocyst stage.CONCLUSION: Using TLC, we observed the fertilization of an ovum by a single spermatozoon, followed by early cleavages, formation of the morula, blastocyst hatching, changes in the embryonic plates, and the development of monozygotic twins from the incubated blastocysts.