Gas chromatography-mass spectrometry characteristics of methylated perfluoroacyl derivatives of cytosine and 5-methylcytosine.
Gas chromatography-mass spectrometry characteristics of methylated perfluoroacyl derivatives of cytosine and 5-methylcytosine.
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胞嘧啶和5-甲基胞嘧啶甲基化全氟酰基衍生物的气相色谱-质谱特征。
DOI：
10.1016/s0021-9673(01)97735-0
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Vouros,P
中科院分区：
文献类型：
--
作者：
Mohamed,GB;Nazareth,A;Hayes,MJ;Giese,RW;Vouros,P
五氟苯甲酰基和七氟丁酰基衍生物的胞嘧啶和五氟苯甲酰基-5-甲基胞嘧啶已通过质谱检查后，全甲基化。用电子轰击质谱确认了衍生物的结构。全甲基化的五氟苯甲酰基胞嘧啶和5-甲基胞嘧啶衍生物的毛细管气相色谱-质谱定量分析是敏感的。在负离子化学电离条件下，可检测到1毫微微克。
Pentafluorobenzoyl and heptafluorobutyryl derivatives of cytosine and pentafluorobenzoyl-5-methylcytosine have been examined by mass spectrometry following permethylation. Electron impact mass spectra were used to confirm the structures of the derivatives. The permethylated pentafluorobenzoyl derivatives of cystosine and 5-methylcytosine are sensitive for quantitative analysis by capillary gas chromatography—mass specrometry. Under negative ion chemical ionization conditions 1 femtogram of each is detected.