In situ immunological determination of basic carbohydrate structures of gangliosides on thin-layer plates.
In situ immunological determination of basic carbohydrate structures of gangliosides on thin-layer plates.
复制标题
薄层板上神经节苷脂基本碳水化合物结构的原位免疫学测定。
DOI：
10.1016/0003-2697(85)90627-x
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发表时间：
1985
影响因子：
2.9
通讯作者：
Yu,RK
中科院分区：
文献类型：
--
作者：
Saito,M;Kasai,N;Yu,RK
本文建立了一种用薄层层析免疫染色技术测定神经节苷脂基本糖结构的免疫学方法。在神经节苷脂的高效薄层色谱法之后，用0.4%聚甲基丙烯酸异丁酯溶液处理色谱图。然后将产脲节杆菌神经氨酸酶应用于层析板上原位分离的神经节苷脂。该程序将从核心寡糖骨架中去除外部和内部唾液酸残基。然后将所得糖脂产物依次与抗-Gg 4血清和125 I-葡萄球菌蛋白A孵育，并暴露于X射线胶片。通过高度特异性结合，抗Gg 4抗体仅检测具有Gg 4寡糖骨架的神经节苷脂。
An immunological method for the determination of the basic carbohydrate structure of gangliosides by using a thin-layer chromatographic immunostaining technique was developed. After high-performance thin-layer chromatography of gangliosides, the chromatogram is treated with a 0.4% polyisobutylmethacrylate solution. Arthrobacter ureafaciens neuraminidase is then applied to the separated gangliosides in situ on the chromatographic plate. This procedure will remove both external and internal sialic acid residues from the core oligosaccharide backbone. The resulting glycolipid products are then incubated with anti-Gg4 serum and125I-staphylococcal protein A, successively, and exposed to an X-ray film. Through a highly specific binding, the anti-Gg4 antibody detects only those gangliosides having the oligosaccharide backbone of Gg4.