Transposition of IS1181 in the genomes of Staphylococcus and Listeria
Transposition of IS1181 in the genomes of Staphylococcus and Listeria
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DOI：
10.1111/j.1574-6968.1999.tb13718.x
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发表时间：
1999-08-01
影响因子：
2.1
通讯作者：
El Solh, N
中科院分区：
文献类型：
--
作者：
Chesneau, O;Lailler, R;El Solh, N
构建重组质粒pIP 1713，分析插入序列IS 1181在金黄色葡萄球菌RN 4220、肉葡萄球菌TM 300和单核细胞增生李斯特菌EGD中的转座活性。该11.3-kb质粒含有两个遗传上不同的元件：（i）pE 194 ts衍生的复制子，其ermC基因赋予几种革兰氏阳性细菌对红霉素的抗性，和（ii）从S. aureus BM 3121，其中四环素抗性基因泰特（T）已插入转座酶编码基因和下游反向重复序列之间。当通过电穿孔导入三种细菌宿主时，pIP 1713将IS 1181 Omega泰特（T）递送到不同的染色体位点。pIP 1713与宿主染色体之间偶尔检测到共整合结构。转座与插入位点处的8-bp重复相关。IS 1181 Omega泰特（T）可用于革兰氏阳性菌的随机诱变。(C)1999年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
The recombinant plasmid pIP1713 was constructed to analyse the transpositional activity of the insertion sequence IS1181 in Staphylococcus aureus RN4220, Staphylococcus carnosus TM300 and Listeria monocytogenes EGD. This 11.3-kb plasmid contains two genetically different elements: (i) a pE194ts-derived replicon, the ermC gene of which confers resistance to erythromycin in Gram-positive bacteria of several species, and (ii) a copy of IS1181, cloned from S. aureus BM3121, in which the tetracycline resistance gene, tet(T), has been inserted between the transposase-encoded gene and the downstream inverted repeat. When introduced by electroporation into the three bacterial hosts, pIP1713 delivered IS1181 Omega tet(T) to various chromosomal sites. Cointegrate structures between pIP1713 and the host chromosome were occasionally detected. Transposition was associated with 8-bp repeats at the insertion sites. IS1181 Omega tet(T) could be used for random mutagenesis in Gram-positive bacteria. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.