Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel
Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel
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DOI：
10.1038/s41598-019-44067-9
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发表时间：
2019-05-21
影响因子：
4.6
通讯作者：
Akiyama, Taishin
中科院分区：
文献类型：
--
作者：
Horie, Kenta;Sasanuma, Hiroki;Akiyama, Taishin
Secondary lymphoid organs are critical for regulating acquired immune responses. The aim of this study was to characterize the impact of spaceflight on secondary lymphoid organs at the molecular level. We analysed the spleens and lymph nodes from mice flown aboard the International Space Station (ISS) in orbit for 35 days, as part of a Japan Aerospace Exploration Agency mission. During flight, half of the mice were exposed to lg by centrifuging in the ISS, to provide information regarding the effect of microgravity and lg exposure during spaceflight. Whole-transcript cDNA sequencing (RNA-Seq) analysis of the spleen suggested that erythrocyte-related genes regulated by the transcription factor GATA1 were significantly down-regulated in ISS-flown vs. ground control mice. GATA1 and Tall (regulators of erythropoiesis) mRNA expression was consistently reduced by approximately half. These reductions were not completely alleviated by lg exposure in the ISS, suggesting that the combined effect of space environments aside from microgravity could down-regulate gene expression in the spleen. Additionally, plasma immunoglobulin concentrations were slightly altered in ISS-flown mice. Overall, our data suggest that spaceflight might disturb the homeostatic gene expression of the spleen through a combination of microgravity and other environmental changes.
