Urine-based Detection of Congenital Portosystemic Shunt in C57BL/6 Mice.
Urine-based Detection of Congenital Portosystemic Shunt in C57BL/6 Mice.
复制标题
C57BL/6小鼠先天性门体分流的尿液检测
DOI：
10.1093/function/zqad040
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发表时间：
2023
期刊：
影响因子：
6.2
通讯作者：
Vijay-Kumar, Matam
中科院分区：
文献类型：
--
作者：
Yeoh, Beng San;Golonka, Rachel M.;Saha, Piu;Kandalgaonkar, Mrunmayee R.;Tian, Yuan;Osman, Islam;Patterson, Andrew D.;Gewirtz, Andrew T.;Joe, Bina;Vijay-Kumar, Matam
广泛用于生物医学研究的 C57BL/6 J 小鼠中，先天性门体分流 (PSS) 的偶发发生率约为十分之一，导致血清学、代谢和生理参数异常。因此，患有 PSS 的小鼠应被识别为研究中的异常值。因此，我们寻求可靠且有效地识别 PSS 小鼠的方法。血清总胆汁酸 ≥ 40 µm 是小鼠 PSS 的真正生物标志物，但该生物标志物的实用性受到其成本和侵入性的限制，特别是在要筛选大量小鼠的情况下。这促使我们研究尿液检测是否可以作为 PSS 诊断的一种简单、廉价、无创的方法。代谢组分析发现，PSS 小鼠尿液中的克雷布斯循环中间体（即柠檬酸盐、α-酮戊二酸盐和富马酸盐）显着升高。我们利用此类代谢物的铁螯合和降低 pH 特性作为 3 种基于尿液的 PSS 筛查测试的基础：尿铁螯合测定、pH 试纸测试和酚红测定。我们的研究结果证明了使用这些比色测定法的可行性，通过直接观察可以评估其读数，以廉价、快速和无创的方式诊断 PSS。应用我们的尿液 PSS 筛查方案可以通过对 PSS 小鼠进行分层来帮助生物医学研究，目前，这可能会混淆许多正在进行的研究。尿液分析检测小鼠门体分流。
Sporadic occurrence of congenital portosystemic shunt (PSS) at a rate of ∼1 out of 10 among C57BL/6 J mice, which are widely used in biomedical research, results in aberrancies in serologic, metabolic, and physiologic parameters. Therefore, mice with PSS should be identified as outliers in research. Accordingly, we sought methods to, reliably and efficiently, identify PSS mice. Serum total bile acids ≥ 40 µm is a bona fide biomarker of PSS in mice but utility of this biomarker is limited by its cost and invasiveness, particularly if large numbers of mice are to be screened. This led us to investigate if assay of urine might serve as a simple, inexpensive, noninvasive means of PSS diagnosis. Metabolome profiling uncovered that Krebs cycle intermediates, that is, citrate, α-ketoglutarate, and fumarate, were strikingly and distinctly elevated in the urine of PSS mice. We leveraged the iron-chelating and pH-lowering properties of such metabolites as the basis for 3 urine-based PSS screening tests: urinary iron-chelation assay, pH strip test, and phenol red assay. Our findings demonstrate the feasibility of using these colorimetric assays, whereby their readout can be assessed by direct observation, to diagnose PSS in an inexpensive, rapid, and noninvasive manner. Application of our urinary PSS screening protocols can aid biomedical research by enabling stratification of PSS mice, which, at present, likely confound numerous ongoing studies. Urinalysis to detect portosystemic shunt in mice.
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影响因子：
3.7
作者：
Ronda OAHO;van de Heijning BJM;de Bruin A;Thomas RE;Martini I;Koehorst M;Gerding A;Koster MH;Bloks VW;Jurdzinski A;Mulder NL;Havinga R;van der Beek EM;Reijngoud DJ;Kuipers F;Verkade HJ
通讯作者：
Verkade HJ
影响因子：
64.5
作者：
Singh V;Yeoh BS;Chassaing B;Xiao X;Saha P;Aguilera Olvera R;Lapek JD Jr;Zhang L;Wang WB;Hao S;Flythe MD;Gonzalez DJ;Cani PD;Conejo-Garcia JR;Xiong N;Kennett MJ;Joe B;Patterson AD;Gewirtz AT;Vijay-Kumar M
通讯作者：
Vijay-Kumar M
影响因子：
13.5
作者：
Glantz, A;Marschall, HW;Mattsson, LÅ
通讯作者：
Mattsson, LÅ
DOI：
10.1073/pnas.190256997
发表时间：
2000-09-12
影响因子：
11.1
作者：
Lahvis, GP;Lindell, SL;Bradfield, CA
通讯作者：
Bradfield, CA
DOI：
10.1152/ajpendo.00332.2001
发表时间：
2002-04-01
影响因子：
5.1
作者：
Burcelin, M;Crivelli, V;Thorens, B
通讯作者：
Thorens, B