Effects of 2,2′,5,5′-tetrachlorobiphenyl and biphenyl on cell membranes of Ralstonia eutropha H850
Effects of 2,2′,5,5′-tetrachlorobiphenyl and biphenyl on cell membranes of Ralstonia eutropha H850
复制标题
DOI：
10.1111/j.1574-6968.2001.tb10686.x
复制
发表时间：
2001-06-12
影响因子：
2.1
通讯作者：
Trevors, JT
中科院分区：
文献类型：
--
作者：
Kim, IS;Lee, H;Trevors, JT
以1,6-二苯基-1,3,5-己三烯（DPH）为探针，通过荧光偏振测量，研究了2,2',5,5'-四氯联苯（TcCB）和联苯对真菌性Ralstonia Eutropha H850细胞质膜的影响，并测定了细胞脂肪酸组成。TeCB通过降低dpi - 1荧光偏振，显著影响了在果糖作用下生长的真核酵母H850细胞的膜。相比之下，在联苯上生长的细胞，TeCB对膜极化的影响明显小于在果糖上生长的细胞。在联苯上生长的细胞中，总饱和脂肪酸与不饱和脂肪酸的比例增加，表明TeCB对这些细胞膜的流化作用较小。当联苯培养的细胞被转移回果糖培养基时，它们需要25代才能使这些细胞的膜极化和脂肪酸组成恢复到最初的果糖培养的细胞。对温度变化的重新适应只需要五代就能恢复正常。这些结果表明，联苯影响细胞的方式比简单地流化细胞质膜多。(C) 2001年欧洲微生物学会联合会。Elsevier Science B.V.版权所有。
The effects of 2,2',5,5'-tetrachlorobiphenyl (TcCB), a PCB congener, and biphenyl on the cytoplasmic membranes of Ralstonia Eutropha H850 were investigated by measuring fluorescence polarization using 1,6-diphenyl-1,3,5-hexatriene (DPH) as the probe, and determining the cellular fatty acid compositions. TeCB significantly affected the membrane of R. eutropha H850 cells grown on fructose by decreasing DPI-I fluorescence polarization. In contrast, the membrane of cells grown on biphenyl showed a considerably less significant effect of TeCB on membrane polarization than in fructose-grown cells. An increase in the ratio of total saturated to unsaturated fatty acids in cells grown on biphenyl suggested less of a fluidizing effect of TeCB on membranes in those cells. When biphenyl-grown cells were transferred back to a fructose medium, they required 25 generations for the membrane polarization and fatty acid compositions of these cells to revert back to those of the initial fructose-grown cells. The re-adaptation to a change in temperature required only five generations to return to normal. These results show that biphenyl affects cells in more ways than simply fluidizing the cytoplasmic membrane. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.