FVB/N - AN INBRED MOUSE STRAIN PREFERABLE FOR TRANSGENIC ANALYSES
FVB/N - AN INBRED MOUSE STRAIN PREFERABLE FOR TRANSGENIC ANALYSES
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DOI：
10.1073/pnas.88.6.2065
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发表时间：
1991-03-01
影响因子：
11.1
通讯作者：
OVERBEEK, PA
中科院分区：
文献类型：
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作者：
TAKETO, M;SCHROEDER, AC;OVERBEEK, PA
FVB/N小鼠为大多数转基因实验和随后的遗传分析提供了一个合适的系统。近交系FVB/N品系的特征在于旺盛的繁殖性能和一贯的大窝仔。此外，受精的FVB/N卵含有大而突出的原核，这有利于DNA的显微注射。受精卵中大原核的表型是与FVB/N卵母细胞相关的显性性状，但与FVB/N精子无关。在产生转基因小鼠的实验中，将相同的DNA构建体注射到三种不同类型的受精卵中：FVB/N、C57 BL/6 J和（C57 BL/6 J X SJL/J）F1。FVB/N受精卵在注射后存活良好，获得的转基因动物的效率与F1受精卵相似，但远优于C57 BL/6 J受精卵。对FVB/N株系进行了15条染色体44个基因座的遗传标记分析，并与常用的自交系进行了比较。除了白化病FVB/N菌株之外，正在构建FVB/N的色素同源菌株。这些特征使得FVB/N菌株有利于用于转基因小鼠的研究。
FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J X SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice.