Shrimp Taura syndrome virus:: genomic characterization and similarity with members of the genus Cricket paralysis-like viruses
Shrimp Taura syndrome virus:: genomic characterization and similarity with members of the genus Cricket paralysis-like viruses
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DOI：
10.1099/0022-1317-83-4-915
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发表时间：
2002-04-01
影响因子：
3.8
通讯作者：
Bonami, JR
中科院分区：
文献类型：
--
作者：
Mari, J;Poulos, BT;Bonami, JR
Taura综合征病毒（TSV）的单链基因组RNA长度为10205个核苷酸（不包括3' poly(A)尾），包含两个大的开放阅读框（orf），由207个核苷酸的基因间区隔开。orf的两侧是377个核苷酸的5‘非翻译区（UTR）和226个核苷酸的3’非翻译区，后面是一个聚(a)尾。预测的ORF1氨基酸序列揭示了解旋酶、蛋白酶和RNA依赖的RNA聚合酶的序列基序特征，与几种植物和动物RNA病毒的非结构蛋白相似。此外，位于ORF1 n端区域的短氨基酸序列与来自双链DNA病毒和动物的凋亡抑制蛋白的杆状病毒IAP重复（BIR）结构域具有显著的相似性。这是在单链RNA病毒中首次报道的birb样序列，但其功能尚不清楚。三种TSV衣壳蛋白（55、40和24 kDa）的n端氨基酸序列在ORF2中被定位，ORF2与ORF1不在同一阅读框中，并且在结构基因上游具有一个AUG密码子。然而，基因间区显示与板球麻痹样病毒属的核苷酸序列相似，表明类似的非aug介导的翻译机制。TSV基因组的结构[5' utr -非结构蛋白-基因间utr -结构蛋白-3' UTR-poly(A) tail]与感染昆虫的小RNA病毒的结构相似，这些病毒最近被重新组合成一个新的病毒属——蟋蟀麻痹样病毒。
The single-stranded genomic RNA of Taura syndrome virus (TSV) is 10205 nucleotides in length, excluding the 3' poly(A) tail, and contains two large open reading frames (ORFs) that are separated by an intergenic region of 207 nucleotides. The ORFs are flanked by a 377 nucleotide 5' untranslated region (UTR) and a 226 nucleotide 3' UTR followed by a poly(A) tail. The predicted amino acid sequence of ORF1 revealed sequence motifs characteristic of a helicase, a protease and an RNA-dependent RNA polymerase, similar to the non-structural proteins of several plant and animal RNA viruses. In addition, a short amino acid sequence located in the N-terminal region of ORF1 presented a significant similarity with a baculovirus IAP repeat (BIR) domain of inhibitor of apoptosis proteins from double-stranded DNA viruses and from animals. The presence of this BIR-like sequence is the first reported in a single-stranded RNA virus, but its function is unknown. The N-terminal amino acid sequence of three TSV capsid proteins (55, 40 and 24 kDa) were mapped in ORF2, which is not in the same reading frame as ORF1 and possesses an AUG codon upstream of the structural genes. However, the intergenic region shows nucleotide sequence similarity with those of the genus Cricket paralysis-like viruses, suggesting a similar non-AUG-mediated translation mechanism. The structure of the TSV genome [5' UTR-non-structural proteins-intergenic UTR-structural proteins-3' UTR-poly(A) tail] is similar to those of small insect-infecting RNA viruses, which were recently regrouped into a new virus genus, Cricket paralysis-like viruses.