SEPARATE REGULATORY ELEMENTS ARE RESPONSIBLE FOR THE COMPLEX PATTERN OF TISSUE-SPECIFIC AND DEVELOPMENTAL TRANSCRIPTION OF THE YELLOW LOCUS IN DROSOPHILA-MELANOGASTER
SEPARATE REGULATORY ELEMENTS ARE RESPONSIBLE FOR THE COMPLEX PATTERN OF TISSUE-SPECIFIC AND DEVELOPMENTAL TRANSCRIPTION OF THE YELLOW LOCUS IN DROSOPHILA-MELANOGASTER
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DOI：
10.1101/gad.1.9.996
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发表时间：
1987-11-01
影响因子：
10.5
通讯作者：
CORCES, VG
中科院分区：
文献类型：
--
作者：
GEYER, PK;CORCES, VG
通过对携带不同体外修饰的黄色基因的生殖系转化体的表型分析，鉴定了参与控制果蝇黄色基因座发育和空间表达的DNA序列。位于-2873和-1868之间以及-1868和-700之间的两个区域分别作为组织特异性增强子，分别调节成虫翅膀和身体中的黄色转录。位于更接近mRNA帽位置的序列，从转录开始起在-225bp到-91bp之间，负责在幼虫发育过程中牙齿带和口腔部分的黄色表达。最后，成虫刷毛的颜色由黄色基因内含子上的序列调节。这些结果表明，存在几个独立的DNA元件，这些DNA元件负责黄色基因座不同的时空表达模式。
DNA sequences involved in the control of the developmental and spatial expression of the yellow locus of Drosophila were identified by phenotypic analysis of germline transformants carrying various in vitro modified yellow genes. Two regions, located between -2873 bp and -1868 bp and between -1868 bp and -700 bp, act as tissue-specific enhancers which respectively regulate yellow transcription in the wings and body of the adult flies. Sequences situated closer to the mRNA cap site, between -225 bp and -91 bp from the start of transcription, are responsible for yellow expression in the denticle belts and mouth parts during larval development. Finally, coloration of the adult bristles is regulated by sequences located in the intron of the yellow gene. These results indicate the existence of several separate DNA elements responsible for the different patterns of temporal and spatial expression of the yellow locus.