Restoration of Scarred Vocal Folds Using 5 Amino Acid-Deleted Type Hepatocyte Growth Factor
Restoration of Scarred Vocal Folds Using 5 Amino Acid-Deleted Type Hepatocyte Growth Factor
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DOI：
10.1002/lary.24413
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发表时间：
2014-03-01
期刊：
影响因子：
2.6
通讯作者：
Ito, Juichi
中科院分区：
文献类型：
--
作者：
Mizuta, Masanobu;Hirano, Shigeru;Ito, Juichi
目的/假设我们以前的研究证明了重组人肝细胞生长因子（HGF）对声带瘢痕的再生作用，使用全长HGF。然而，由于缺乏全长HGF的良好生产规范（GMP），尚未实现临床应用。人HGF的另一种天然形式，即5个氨基酸缺失型HGF（dHGF），是根据GMP程序新生产的。在目前的研究中，我们调查了dHGF的效果相比，全长肝细胞生长因子用于治疗声带疤痕使用犬model.Study DesignProspective animal experiments.MethodsThe声带9比格犬单侧受伤。损伤后4周，声带内注射全长HGF（全HGF组）、dHGF（dHGF组）或磷酸盐缓冲盐水（假手术组）。振动和组织学检查进行每组6个月后injure.ResultsVibratory检查显示出显着较低的发声阈值压力和较高的比率，正常化的粘膜波振幅在两个完整的HGF和dHGF组相比，假手术组。组织学检查显示，与假手术组相比，完全HGF和dHGF组的透明质酸均得到恢复。无显着差异，观察到每个参数之间的完整的HGF组和dHGF group.Conclusions dHGF表现出相同的潜力声带瘢痕的再生效果全长HGF。dHGF应适用于声带瘢痕患者的人体临床试验。
Objectives/Hypothesis Our previous studies demonstrated a regenerative effect of recombinant human hepatocyte growth factor (HGF) on vocal fold scarring using full-length HGF. However, clinical application has not yet been achieved because of the lack of a good manufacturing practice (GMP) for full-length HGF. Another natural form of human HGF, 5 amino acid-deleted type HGF (dHGF), has been newly produced under a GMP procedure. In the current study, we investigated the effect of dHGF in comparison with full-length HGF for the treatment of vocal fold scars using a canine model.Study DesignProspective animal experiment.MethodsThe vocal folds of nine beagles were unilaterally injured. Four weeks after injury, the vocal folds were treated with an intracordal injection of full-length HGF (full HGF group), dHGF (dHGF group), or phosphate-buffered saline (sham group). Vibratory and histological examinations were performed for each group 6 months after injury.ResultsVibratory examinations demonstrated significantly lower phonation threshold pressure and a higher ratio of normalized mucosal wave amplitude in both the full HGF and dHGF groups as compared to the sham group. Histological examination showed restoration of hyaluronic acid in both the full HGF and dHGF groups as compared to the sham group. No significant differences were observed for each parameter between the full HGF group and the dHGF group.Conclusions dHGF showed the same potential for regenerative effects on vocal fold scars as full-length HGF. dHGF should be applicable for human clinical trials in patients with vocal fold scars.