Prostaglandin receptors in lower segment myometrium during gestation and labor
Prostaglandin receptors in lower segment myometrium during gestation and labor
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DOI：
10.1016/s0029-7844(98)00378-0
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发表时间：
1999-01-01
影响因子：
7.2
通讯作者：
Myatt, L
中科院分区：
文献类型：
--
作者：
Brodt-Eppley, J;Myatt, L
目的：目的：探讨妊娠期和分娩期子宫肌层松弛型前列腺素（PG）E-2受体（EP 2）和收缩型PG F受体（FP）表达的变化。方法：采用剖宫产术，从4组产妇（早产[28-36周]或足月[37-41周]，临产或非临产，每组10例）中取出子宫下段肌层。分离子宫肌层RNA，并使用人平滑肌蛋白钙调蛋白和PGEP（2）或FP受体亚型mRNA的特异性引物组进行逆转录-聚合酶链反应（PCR）分析。聚合酶链反应产物在凝胶上电泳并可视化，测量条带强度，并将EP 2和FP受体表达归一化为calponin.Results：反转录PCR产生预期大小的产物，并经限制性内切酶切割确认身份。前列腺素EP 2受体亚型mRNA表达（相对于钙调蛋白mRNA）在早产、无分娩组中显著更高（1.28 +/- 0.22，平均值+/-平均值的标准误[SE]）与术语相比，无劳工团体（0.71 +/- 0.09）（P <0.05 Student-Neuman-Kuels），未临产的患者随胎龄显著下降（R =-0.446，P = 0.001）。与所有其他组相比，足月、未分娩组的前列腺素FP受体亚型mRNA表达显著较少（0.61 ± 0.06）（P <0.02）。前列腺素FP受体表达随孕龄显著下降，患者在劳动力（R =-0.646，P = 0.012）和劳动力在足月显着增加。结论：前列腺素EP 2受体亚型表达的变化是一致的影响，维持静止。足月分娩与FP受体表达的显著增加相关，这与对收缩的影响一致。这两种受体亚型之间的平衡可能介导子宫肌层的收缩力。(C)1999年由美国妇产科医师学会。
Objective: To determine if expression of relaxatory prostaglandin (PG) E-2 receptors (EP2 isoform) and contractile PG F receptors (FP isoform) changes during gestation and with labor in pregnant human myometrium.Methods: Lower segment myometrium was removed at cesarean from four groups of parturients (preterm [28-36 weeks] or term [37-41 weeks], either in or not in labor, ten per group). Myometrial RNA was isolated and used for reverse transcription-polymerase chain reaction (PCR) analysis with specific primer sets for human smooth muscle protein calponin and PGEP(2) or FP receptor isoform mRNA. Polymerase chain reaction products were electrophoresed on gels and visualized, band intensity was measured, and EP2 and FP receptor expression was normalized to calponin.Results: Reverse transcription-PCR yielded products of expected sizes, and restriction enzyme cleavage confirmed identities. Prostaglandin EP2 receptor isoform mRNA expression (relative to calponin mRNA) was significantly greater in the preterm, no labor group (1.28 +/- 0.22, mean +/- standard error of the mean [SE]) compared with the term, no labor group (0.71 +/- 0.09) (P < .05 Student-Neuman-Kuels) and declined significantly with gestational age in patients not in labor (R = -.446, P = .001). Prostaglandin FP receptor isoform mRNA expression was significantly less in the term, no labor group (0.61 +/- 0.06) compared with all others (P < .02 Student-Neuman-Kuels). Prostaglandin FP receptor expression declined significantly with gestational age in patients not in labor (R = -.646, P = .012) and increased significantly with labor at term.Conclusion: Changes in prostaglandin EP2 receptor isoform expression are consistent with influence on maintenance of quiescence. Labor at term is associated with a significant increase in FP receptor expression, consistent with influence on contraction. The balance between the two receptor isoforms might mediate myometrial contractility. (C) 1999 by The American College of Obstetricians and Gynecologists.