Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function.
Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function.
复制标题
Mcm2 和 Mcm3 是对 ARS 活性很重要的两种蛋白质，它们在结构和功能上相关。
DOI：
10.1101/gad.5.6.944
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发表时间：
1991
影响因子：
10.5
通讯作者：
Tye,BK
中科院分区：
文献类型：
--
作者：
Yan,H;Gibson,S;Tye,BK
MCM 2和MCM 3是酿酒酵母中DNA复制起始的重要基因。Mcm 2或Mcm 3缺陷的突变体在表型上非常相似。它们都表现出自主复制序列（ARS）特异性微型染色体维持缺陷，尽管它们的ARS特异性不相同。此外，这些突变体表现出有丝分裂前细胞周期停滞和染色体丢失和重组的增加。遗传学研究表明，这两个MCM基因产物在DNA复制中发挥相互作用或互补作用。mcm 2 -1和mcm 3 -1的双突变体在每个单突变体的允许生长温度（23 ° C）下是无活力的。此外，Mcm 3的过度产生加重了mcm 2 -1突变的有害作用，而Mcm 2的过度产生部分地补充了mcm 3 -1突变。MCM 2编码890个氨基酸的蛋白质，其含有对Mcm 2功能至关重要的推定锌指结构域。Mcm 2与Mcm 3及其它三种蛋白质、S.酿酒酵母的Nda 4和Cdc 21。这些蛋白质缺陷突变体的表型表明它们属于参与DNA复制早期步骤的蛋白质家族。
MCM2 and MCM3 are essential genes believed to play important roles in the initiation of DNA replication in Saccharomyces cerevisiae. Mutants defective in Mcm2 or Mcm3 are remarkably similar in phenotype. They both show an autonomously replicating sequence (ARS)-specific minichromosome maintenance defect, although their ARS specificities are not identical. In addition, these mutants exhibit a premitotic cell cycle arrest and an increase in chromosome loss and recombination. Genetic studies suggest that the two MCM gene products play interacting or complementary roles in DNA replication. Double mutants of mcm2-1 and mcm3-1 are inviable at the permissive growth temperature (23 degrees C) for each of the single mutants. Furthermore, overproduction of Mcm3 accentuates the deleterious effect of the mcm2-1 mutation, whereas overproduction of Mcm2 partially complements the mcm3-1 mutation. MCM2 encodes a protein of 890 amino acids containing a putative zinc-finger domain that is essential for Mcm2 function. Mcm2 shows striking homology to Mcm3 and three other proteins, Cdc46 of S. cerevisiae, and Nda4 and Cdc21 of Schizosaccharomyces pombe. The phenotypes of mutants defective in these proteins suggest that they belong to a protein family involved in the early steps of DNA replication.