SEQUENCE-ANALYSIS AND EXPRESSION PATTERNS DIVIDE THE MAIZE KNOTTED1-LIKE HOMEOBOX GENES INTO 2 CLASSES
SEQUENCE-ANALYSIS AND EXPRESSION PATTERNS DIVIDE THE MAIZE KNOTTED1-LIKE HOMEOBOX GENES INTO 2 CLASSES
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DOI：
10.1105/tpc.6.12.1877
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发表时间：
1994-12-01
期刊：
影响因子：
11.6
通讯作者：
HAKE, S
中科院分区：
文献类型：
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作者：
KERSTETTER, R;VOLLBRECHT, E;HAKE, S
使用knotted1 (kn1) 基因的同源框在玉米中分离出12 个相关序列。 kn1 样基因编码的同源域非常相似，氨基酸同一性范围为 55% 至 89%。精确保守的第三螺旋之外的差异使我们能够将基因分为两类。七个 1 类基因的同源域与 kn1 具有 73% 至 89% 的相同残基。四个 2 类基因与 kn1 具有 55% 至 58% 的相同残基，尽管该类内的保守性大于 87%。通过RNA凝胶印迹分析来分析表达模式。 1 类基因在富含分生组织的组织中高度表达，例如营养分生组织和耳原基。在叶子中未检测到表达。 2 类基因在所有组织中表达，但其中一种基因在根中大量表达。使用重组近交群体绘制基因图谱。我们确定 1 号和 8 号染色体上存在两到三个连锁基因的簇；否则，基因分布在整个基因组中。四对基因在序列和表达模式上都相似，映射在基因组的重复区域内。
The homeobox of the knotted1 (kn1) gene was used to isolate 12 related sequences in maize. The homeodomains encoded by the kn1-like genes are very similar, ranging from 55 to 89% amino acid identity. Differences outside the precisely conserved third helix allowed us to group the genes into two classes. The homeodomains of the seven class 1 genes share 73 to 89% identical residues with kn1. The four class 2 genes share 55 to 58% identical residues with kn1, although the conservation within the class is greater than 87%. Expression patterns were analyzed by RNA gel blot analysis. Class 1 genes were highly expressed in meristem-enriched tissues, such as the vegetative meristem and ear primordia. Expression was not detectable in leaves. The class 2 genes were expressed in all tissues, although one was abundantly expressed in roots. The genes were mapped using recombinant inbred populations. We determined that clusters of two to three linked genes are present on chromosomes 1 and 8; otherwise, the genes are distributed throughout the genome. Four pairs of genes, similar in both sequence and expression patterns, mapped within duplicated regions of the genome.