IN-SITU EXPRESSION OF CYTOKINES AND CELLULAR ADHESION MOLECULES IN THE SKIN OF PATIENTS WITH SYSTEMIC-SCLEROSIS - THEIR ROLE IN EARLY AND LATE DISEASE
IN-SITU EXPRESSION OF CYTOKINES AND CELLULAR ADHESION MOLECULES IN THE SKIN OF PATIENTS WITH SYSTEMIC-SCLEROSIS - THEIR ROLE IN EARLY AND LATE DISEASE
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DOI：
10.1159/000163802
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发表时间：
1993-09-01
期刊：
影响因子：
5
通讯作者：
JIMENEZ, SA
中科院分区：
文献类型：
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作者：
KOCH, AE;KRONFELDHARRINGTON, LB;JIMENEZ, SA
细胞因子和细胞粘附分子 (CAM) 可能在系统性硬化症 (SSc) 的炎症和纤维化过程中发挥作用。我们比较了 12 名 SSc 患者和 14 名正常 (NL) 个体皮肤活检中细胞因子和 CAM 的免疫组织学分布。在 CAM 中，介导白细胞-内皮粘附的血管细胞粘附分子 1 (VCAM-1) 在 SSc 上的表达量高于 NL 内皮和颗粒层。与 NL 颗粒层相比，SSc 中 P-选择素上调。 CD44淋巴细胞归巢受体在SSc和NL之间表现出最显着的差异：其在SSc颗粒层、棘层、淋巴细胞和巨噬细胞上的表达增加。关于细胞因子，与 NL 内皮细胞和成纤维细胞相比，SSc 上的白细胞介素 6 (IL-6) 表达增加。肿瘤坏死因子-α (TNF-α) 反应性在 SSc 中比 NL 颗粒层更普遍，而 IL-8 表达在 SSc 上比 NL 内皮更高。一些 CAM（如 VCAM-1 和 P-选择素）和细胞因子（即 TNF-α 和 IL-8）更常见于 SSc 早期（小于或等于 1 年）的皮肤活检中，而其他 CAM（如 IL-6）在疾病晚期表现出上调。结果表明，某些 CAM 和细胞因子可能在 SSc 的早期炎症阶段和晚期纤维化阶段发挥不同的作用。
Cytokines and cellular adhesion molecules (CAMs) may play a role in the inflammatory and fibrotic processes underlying systemic sclerosis (SSc). We compared the immunohistological distribution of cytokines and CAMs in skin biopsies from 12 SSc patients and 14 normal (NL) individuals. Among CAMs, vascular cell adhesion molecule-1 (VCAM-1), which mediates leukocyte-endothelial adhesion, showed increased expression on SSc versus NL endothelium and stratum granulosum. P-selectin was up-regulated in SSc versus NL stratum granulosum. The CD44 lymphocyte homing receptor showed the most striking differences between SSc and NL: its expression was increased in SSc stratum granulosum, stratum spinosum, on lymphocytes, and macrophages. Regarding cytokines, interleukin-6 (IL-6) expression was increased on SSc versus NL endothelium and fibroblasts. Tumor necrosis factor-alpha(TNF-alpha) reactivity was more prevalent in SSc than NL stratum granulosum, whereas IL-8 expression was higher on SSc compared to NL endothelium. Some CAMs, such as VCAM-1 and P-selectin, and cytokines, namely TNF-alpha and IL-8, were more commonly found in skin biopsies taken from early(less than or equal to 1 year's duration) SSc, while others, such as IL-6, showed up-regulation in the late stage of the disease. The results suggest that certain CAMs and cytokines may play a differential role in both the early, inflammatory, and the late, fibrotic stage of SSc.