Bio-orthogonal click reaction-enabled highly specific in situ cellularization of tissue engineering scaffolds
Bio-orthogonal click reaction-enabled highly specific in situ cellularization of tissue engineering scaffolds
复制标题
生物正交点击反应使组织工程支架具有高度特异性的原位细胞化
DOI：
10.1016/j.biomaterials.2019.119615
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发表时间：
2020
期刊：
影响因子：
14
通讯作者：
Bin Liu
中科院分区：
文献类型：
--
作者：
Duo Mao;Chuangnian Zhang;Kenry;Jing Liu;Xiaoxiao Wang;Binhan Li;Hongyu Yan;Fang Hu;Deling Kong;Zhihong Wang;Bin Liu
组织工程通常利用天然或合成的支架来修复或替换受损组织。然而，由于缺乏生物信号的引导，大多数植入支架的内细胞化程度一直较差。在此，我们展示了一种基于生物正交反应的策略来实现组织工程支架的位置特异性和快速细胞化。合成了dbco修饰的聚氯乙烯-聚乙二醇（PCL-PEG- dbco）聚合物，并通过静电纺丝法制备了PCL-PEG- dbco薄膜。同时，通过代谢糖工程获得叠氮标记的巨噬细胞（N3(+)）。通过一系列的体外动力学和体内表征，发现dbco修饰膜显著提高了N3（+）细胞的选择性捕获效率和存活率。此外，共价偶联对细胞活力和增殖的影响可以忽略不计，表明基于生物正交点击反应的组织工程策略是可行的。总的来说，这项工作显示了anin位置正交点击反应在实现高特异性、高效和持久的支架细胞化方面的优势。我们期望在不久的将来，这一总体策略将在组织工程和再生医学中得到广泛应用和应用。
Tissue engineering generally utilizes natural or synthetic scaffolds to repair or replace damaged tissues. However, due to the lack of guidance of biological signals, most of the implanted scaffolds have always suffered from poorin vivocellularization. Herein, we demonstrate a bio-orthogonal reaction-based strategy to realizein situspecific and fast cellularization of tissue engineering scaffold. DBCO-modified PCL-PEG (PCL-PEG-DBCO) polymer was synthesized and then fabricated into PCL-PEG-DBCO film through electrospinning. Meanwhile, azide-labeled macrophages (N3(+) macrophages) were obtained through metabolic glycoengineering. Through a series ofin vitrodynamic andin vivocharacterization, DBCO-modified films were noted to dramatically increase the selective capture efficiency and survival rate of N3(+) cells. Additionally, there is negligible influence of covalent conjugation on cell viability and proliferation, indicating the feasibility of the bio-orthogonal click reaction-based tissue engineering strategy. Overall, this work shows the advantages of anin situbio-orthogonal click reaction in realizing highly specific, efficient, and long-lasting scaffold cellularization. We anticipate that this general strategy would be widely applicable and useful in tissue engineering and regenerative medicine in the near future.